Intended Use
The MMP-19 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MMP-19 protein expression profile in cells. The kit can be used for measuring the relative amounts of MMP-19 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MMP-19.
Contents of Kit
1.96-Well Cell Culture Clear-Bottom Microplate, 1 Plate
2.10x TBS, 24 ml (10x), Clear
3.Quenching Buffer, 24 ml (1x), Clear
4.Blocking Buffer, 50 ml (1x), Clear
5.15x Wash Buffer, 50 ml (15x), Clear
6.100x Anti-MMP-19 Antibody(Rabbit Polyclonal), 60 μl (100x), Purple
7.100x Anti-GAPDH Antibody (Mouse Monoclonal), 60 μl (100x), Green
8.HRP-Conjugated Anti-Rabbit IgG Antibody, 6 ml (1x), Glass
9.HRP-Conjugated Anti-Mouse IgG Antibody, 6 ml (1x), Glass
10.Primary Antibody Diluent, 12 ml (1x), Clear
11.Ready-to-Use Substrate, 12 ml (1x), Brown
12.Stop Solution, 12 ml (1x), Clear
13.Crystal Violet Solution, 6 ml (1x), Glass
14.SDS Solution, 24 ml (1x), Clear
15.Adhesive Plate Seals, 2 Seals
Storage
Upon receipt, the kit should be stored at 4°C. The un-opened kit will be stable for up to 6 months from the date of shipment if stored at 4°C. Diluted Anti-MMP-19 Antibody and diluted Anti-GAPDH Antibody can each be stored at 4°C for up to two weeks. HRP-Conjugated Anti-Rabbit IgG Antibody and HRP-Conjugated Anti-Mouse IgG Antibody will be stable at 4°C for up to six months. The SDS Solution should be stored at room temperature or warmed up to room temperature if stored at 4°C.
COA
Publication 
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