Sample
Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva
Intended Use
The Human MIA Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human MIA with a 96-well strip plate that is pre-coated with antibody specific for MIA.
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Performance Characteristics
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Detection Range
15.6 pg/ml - 1000 pg/ml
Detection Limit
<10 pg/ml
Standard Curve
The highest O.D. value might be higher or lower than in the example. The experiment result is statistically significant if the highest O.D. value is no less than 1.0.
A standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed

General Notes
Other ELISA Kits
Citations
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Douziech, N; Calvo, E; et al. Activation of MAP kinases in growth responsive pancreatic cancer cells. CELLULAR SIGNALLING 11:591-602(1999).
Reeves, A; Zagurovskaya, M; et al. Inhibition of transforming growth factor-beta signaling in normal lung epithelial cells confers resistance to ionizing radiation. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 68:187-195(2007).