MBD1 Knockout Cell Lysate

Name: MBD1 Knockout Cell Lysate
SKU: DAG-KO205
Rating: 5 (1 reviews)

MBD1 Knockout Cell Lysate from HeLa cell line for anti-MBD1 antibody specificity validation.

COA

SDS

Datasheet

Summary

Product Overview

HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The MBD1 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.

Nature

Cell Lysate

Alternative Names

MBD1; methyl-CpG binding domain protein 1; methyl-CpG-binding domain protein 1; CXXC3; PCM1; CXXC-type zinc finger protein 3; protein containing methyl-CpG-binding domain 1; methyl-CpG binding domain protein 1 isoform PCM1; the regulator of fibroblast growth factor 2 (FGF-2) transcription; RFT;

Application Notes

Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Dilution

Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.

Format

Lyophilized

Concentration

The protein concentration was determined with BCA assay.

Buffer

RIPA buffer

Preservative

None

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Methyl-CpG binding domain 1 gene polymorphisms and lung cancer risk in a Chinese population

BIOMARKERS

Authors: Liu, Hongliang; Jin, Guangfu; Wang, Haifeng; Wu, Wenting; Liu, Yanhong; Qian, Ji; Fan, Weiwei; Ma, Hongxia; Miao, Ruifen; Hu, Zhibin; Sun, Weiwei; Wang, Yi; Jin, Li; Wei, Qingyi; Shen, Hongbing; Huang, Wei; Lu, Daru

Abstract

Polymorphisms of the methyl-CpG binding domain 1 (MBD1) gene may influence MBD1 activity on gene expression profiles, thereby modulating individual susceptibility to lung cancer. To test this hypothesis, we investigated the associations of four MBD1 polymorphisms and lung cancer risk in a Chinese population. Single locus analysis revealed significant associations between two polymorphisms (rs125555 and rs140689) and lung cancer risk (p=0.011 and p=0.005, respectively). Since the two polymorphisms were in linkage disequilibrium, further haplotype analyses were performed and revealed a significant association with lung cancer (global test p-value=0.0041). Our results suggested that MBD1 polymorphisms might be involved in the development of lung cancer. Validation of these findings in larger studies of other populations is needed.

Expression of key genes involved in DNA methylation during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Authors: Stachecka, Joanna; Lemanska, Weronika; Noak, Magdalena; Szczerbal, Izabela

Abstract

The normal course of DNA methylation depends on the correct functioning of the DNA methylation machinery, which include DNA methyltransferase enzymes (DNMTs) and methyl-CpG-binding domain proteins (MBDs). So far, little is known about the activity of these components during adipogenesis in the pig. The aim of this study was to analyze the expression of ten genes (DNMT1, DNMT3a, DNMT3b, MBD1, MBD2, MBD3, MBD4, MeCP2, UHRF1, and CBX5) during in vitro differentiation into adipocytes of porcine mesenchymal stem cells derived from adipose (AD-MSC) and from bone marrow tissue (BM-MSC). We found that, in undifferentiated cells, the global methylation level was higher in BM-MSC than in AD-MSC, but had similar levels in adipocytes. The transcript level of the DNMT1 gene increased at the beginning of adipogenesis and then decreased, while DNMT3a and DNMT3b transcripts increased during differentiation. All the examined MBD genes show similar expression patterns within the studied system (AD-MSC and BM-MSC). The transcript abundance of UHRF1 and CBX5 decreased in both systems. The changes in the expression patterns of these genes points to the dynamic nature of DNA methylation during porcine adipogenesis. (C) 2019 Elsevier Inc. All rights reserved.