Human Endothelial lipase ELISA Kit

Sample

serum, plasma, cell culture supernates, urine, tissue homogenates

Species Reactivity

Human

Intended Use

For the quantitative determination of human endothelial lipase (EL) concentrations in serum, plasma, cell culture supernates, urine, tissue homogenates.
This package insert must be read in its entirety before using this product.

Contents of Kit

1. Assay plate (12 x 8 coated Microwells): 1(96 wells)
Opened kit: May be stored for up to 1 month at 2 - 8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp.

2. Standard (Freeze dried): 2
3. Biotin-antibody (100 x concentrate): 1 x 120 μl
4. HRP-avidin (100 x concentrate): 1 x 120 μl
Opened kit: May be stored for up to 1 month at 2 - 8°C. If don't make recent use, better keep it store at -20°C.

5. Biotin-antibody Diluent: 1 x 15 ml
6. HRP-avidin Diluent: 1 x 15 ml
7. Sample Diluent: 1 x 50 ml
8. Wash Buffer (25 x concentrate): 1 x 20 ml
9. TMB Substrate: 1 x 10 ml
10. Stop Solution: 1 x 10 ml
Opened kit: May be stored for up to 1 month at 2 - 8°C.

11. Adhesive Strip (For 96 wells): 4
12. Instruction manual: 1

Storage

Unopened kit: Store at 2 - 8°C. Do not use the kit beyond the expiration date.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

0.625 ng/ml-40 ng/ml.

Sensitivity

The minimum detectable dose of human EL is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

General Description

This protein has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family.

Standard Curve