Keratin 17 ELISA Kit

Name: Keratin 17 ELISA Kit
SKU: DEIA-XYA1097
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

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SDS

Datasheet

Key features and details

The Colorimetric Cell-Based ELISA Kit enables the detection of target proteins and the effects of stimulation conditions on their expression in different cell lines. The assay is based on an indirect ELISA format where target proteins are captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind to the Fc region of the primary antibody. The HRP enzyme catalyzes a colorimetric reaction upon substrate addition, which can be terminated with Stop Solution, allowing for the measurement of absorbance and determination of protein concentration.
The Keratin 17 Cell-Based ELISA Kit is a convenient, lysate-free, high-throughput, and sensitive assay that can monitor target protein Keratin 17 and its expression profile in cells.

Sample

Cultured cells

Species Reactivity

Human, Mouse, Rat

Detection Method

ELISA

Intended Use

The Keratin 17 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Keratin17 protein expression profile in cells. The kit can be used for measuring the relative amounts of Keratin17 in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Keratin17.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 1 Plate.
2. 10x TBS: 24 mL (10x), Clear.
3. Quenching Buffer, 24 mL (1x), Clear.
4. Blocking Buffer, 50 mL (1x), Clear.
5. 15x Wash Buffer, 50 mL (10x), Clear.
6. 100x Anti-Keratin 17 Antibody (Rabbit Polyclonal), 60 μL (100x), Purple.
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal), 60 μL (100x), Green.
8. HRP-Conjugated Anti-Rabbit IgG Antibody, 6 mL (1x), Glass.
9. HRP-Conjugated Anti-Mouse IgG Antibody, 6 mL (1x), Glass.
10. Primary Antibody Diluent, 12 mL (1x), Clear.
11. Ready-to-Use Substrate, 12 mL (1x), Brown.
12. Stop Solution, 12 mL (1x), Clear.
13. Crystal Violet Solution, 6 mL (1x), Glass.
14. SDS Solution, 24 mL (1x), Clear.
15. Adhesive Plate Seals, 4 Seals.

Storage

2-8°C

General Description

The kit is designed for research purposes only and is not intended for diagnostic or clinical procedures. It allows for the detection of Keratin 17 in various cell lines and can be used to measure the effects of different treatments, inhibitors, or activators on Keratin 17 expression.

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Customer Reviews

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Alterations of keratins, involucrin and filaggrin gene expression in canine atopic dermatitis

RESEARCH IN VETERINARY SCIENCE

Authors: Theerawatanasirikul, Sirin; Sailasuta, Achariya; Thanawongnuwech, Roongroje; Suriyaphol, Gunnaporn

Abstract

Canine atopic dermatitis (CAD) is a common allergic skin disease in dogs, associated with a defective epidermal barrier. In this study we investigated the alterations in skin keratinocyte proliferation and differentiation in CAD by quantitative reverse transcription-polymerase chain reaction. Gene expression of keratin (KRT) markers of proliferative and differentiated keratinocytes, together with that of cornified envelope proteins, involucrin (IVL) and filaggrin (FLG), were evaluated. An upregulation of KRT5 and KRT17 in both lesional and non-lesional AD skin was observed (p < 0.05) whereas KRT2e, KRT14, IVL and FLG expression were significantly increased only in lesional AD skin (p < 0.05). Additionally, the expression levels of KRT5, KRT14, KRT17 and IVL in CAD were strongly correlated. In conclusion, the expression of the majority of the studied keratins, as well as IVL and FLG is increased in CAD with close correlation between the proliferative keratins. This is the first report of a correlation of KRT and IVL genes with CAD. (c) 2012 Elsevier Ltd. All rights reserved.

Reassessment of microarray expression data of porokeratosis by quantitative real-time polymerase chain reaction

JOURNAL OF CUTANEOUS PATHOLOGY

Authors: Zhang, Zheng-Hua; Wang, Zhi-Min; Crosby, Meredith E.; Kang, Ke-Fei; Luan, Jing; Huang, Wei; Xiang, Lei-Hong; Zheng, Zhi-Zhong

Abstract

Background: Porokeratosis (PK) is a heterogeneous group of keratinization disorders that exhibit similarities with psoriasis at both the clinical and molecular levels. Methods: The transcript levels of keratin (KRT) 6A, 16, 17, S100A7, A8, A9, p53 and three candidate genes (i.e. SART3, SSH1 and ARPC3) were reassessed in pairwise lesional and uninvolved skin from nine patients with PK by real-time quantitative polymerase chain reaction (RTQ-PCR). Results: The results of RTQ-PCR confirmed that KRT6A, 16, S100A7, A8 and A9 (p = 0.008) were mostly up-regulated in the lesional skin when compared with uninvolved skin. Different from the microarray data, there was no significant difference observed in KRT17 expression patterns between lesional and normal-appearing skin (p = 0.066). No statistical difference was observed in p53 and three candidate genes' expression patterns between lesional and uninvolved skin. Conclusions: In the present study, 9 of the 10 gene expression measured by RTQ-PCR in PK were statistically comparable to microarray data. KRT6A was identified as specific biomarker for porokeratotic keratinocytes, as it was the most significantly up-regulated gene in the nine patient samples.