Intended Use
The Keratin 17 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Keratin17 protein expression profile in cells. The kit can be used for measuring the relative amounts of Keratin17 in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Keratin17.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 Plate.
2. 10x TBS: 24 mL (10x), Clear.
3. Quenching Buffer, 24 mL (1x), Clear.
4. Blocking Buffer, 50 mL (1x), Clear.
5. 15x Wash Buffer, 50 mL (10x), Clear.
6. 100x Anti-Keratin 17 Antibody (Rabbit Polyclonal), 60 μL (100x), Purple.
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal), 60 μL (100x), Green.
8. HRP-Conjugated Anti-Rabbit IgG Antibody, 6 mL (1x), Glass.
9. HRP-Conjugated Anti-Mouse IgG Antibody, 6 mL (1x), Glass.
10. Primary Antibody Diluent, 12 mL (1x), Clear.
11. Ready-to-Use Substrate, 12 mL (1x), Brown.
12. Stop Solution, 12 mL (1x), Clear.
13. Crystal Violet Solution, 6 mL (1x), Glass.
14. SDS Solution, 24 mL (1x), Clear.
15. Adhesive Plate Seals, 4 Seals.
General Description
The kit is designed for research purposes only and is not intended for diagnostic or clinical procedures. It allows for the detection of Keratin 17 in various cell lines and can be used to measure the effects of different treatments, inhibitors, or activators on Keratin 17 expression.
COA
Publication 
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