Anti-IgY h+l Antibody polyclonal antibody

Objective: It has long been known that chickens, like mammals, are capable of producing antigen-specific immunoglobulin Y (IgY), which functions similar to IgG. The present study was performed to investigate the activity of IgY anti-Mycobacterium tuberculosis on proliferation, interleukin (IL)-2, and interferon (IFN)-gamma expression of rat peripheral blood mononuclear cells (PBMCs). Materials and Methods: The activity of IgY anti-M. tuberculosis in different doses (25, 50, and 100 mu g/ml) on rat PBMCs proliferation was determined by 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide assay. The production of IL-2 and IFN-gamma in the PBMC supernatant was determined using enzyme-linked immunosorbent assay. Investigation was performed on mRNA expression of IL-2 and IFN-gamma by reverse transcription-polymerase chain reaction (RT-PCR). Results: IgY anti-M. tuberculosis significantly increased the proliferation of rat PBMC. Furthermore, IgY anti-M. tuberculosis dose dependently increased IL-2 and IFN-gamma production in PBMC, suggesting that pharmacological activities of IgY anti-M. tuberculosis in PBMC may be mediated by regulating the production of cytokines. In the RT-PCR, expression of cytokines such as IL-2 and IFN-gamma in PBMC cultures was increased by IgY anti-M. tuberculosis. Conclusions: We concluded that increasing IL-2 and IFN-gamma productions in PBMC was related to IgY anti-M. tuberculosis, stimulating the mRNA transcription (gene expression) of these cytokines which can induce proliferation of PBMC.

Fowl cholera affects the poultry farming including ducks. The commercial fowl cholera vaccines using parenteral administration are available. Recently, an intranasal fowl cholera vaccines have been developed and tested in layers. This study, we analyzed the biological function of recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 and its antiserum. In addition, we also evaluated the protective efficacy in Khaki Campbell ducks. An adhesion inhibition assay on duck embryo fibroblast (DEF) cells was performed demonstrating that rOmpH-immunized duck sera had a potential inhibitory effect on adhesion ability of bacterial strain. An intranasal fowl cholera vaccine was formulated containing 100 mu g rOmpH and 3 mu g E. coli enterotoxin B (LTB) as an adjuvant. Ducks were intranasally immunized three times at three-week intervals. Challenge exposure was conducted by inoculation at 3.5 x 10(3) CFU/ml of a strain of X-73 at four weeks after the last immunization. Sera IgY and secretory IgA antibody titers were significantly increased (P < 0.05) post immunization. Lymphocytes from ducks immunized with the rOmpH-LTB-based intranasal vaccine showed higher proliferative response to P. multocida antigens than those from ducks immunized with only rOmpH or LTB (P < 0.05). Protection conferred by immunization with an intranasal or bacterin vaccine in ducks against challenge-exposure were 90% and 80%, respectively. We conclude that the intranasal fowl cholera vaccine protected ducks from artificial P. multocida infection. However, the rOmpH will be formulated with the commercial adjuvant and will be conducted against more P. multocida field strains in the duck flocks.