IRS-1 (Phospho-Ser323) ELISA Kit

Name: IRS-1 (Phospho-Ser323) ELISA Kit
SKU: DEIA-XYA1866
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

cultured cells

Species Reactivity

Human; Mouse; Rat

Intended Use

The IRS-1 (Phospho-Ser323) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IRS-1 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated IRS-1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IRS-1 phosphorylation.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 2 Plates
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-IRS-1 (Phospho-Ser323) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-IRS-1 Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x), Clear
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x), Clear
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x), Clear
16. Adhesive Plate Seals: 4 Seals

Storage

4°C/6 Months

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Activation of Wnt/beta-catenin pathway causes insulin resistance and increases lipogenesis in HepG2 cells via regulation of endoplasmic reticulum stress

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Authors: Lei, Zili; Yang, Lanxiang; Yang, Yanhong; Yang, Jing; Niu, Zhenpeng; Zhang, Xueying; Song, Qi; Lei, Yuting; Wu, Huijuan; Guo, Jiao

Abstract

Background: Wnt/beta-catenin signaling is involved in glucose and lipid metabolism, but the mechanism is not clear yet. Aim: The objective is to study mechanisms of Wnt/beta-catenin signaling on regulating hepatocytes metabolism. Methods: Real-time qPCR, Western blot, and Oil-red O staining methods were used. Results: The Wnt/beta-catenin signaling was activated in hepatocytes by CP21R7, and the level of phosphorylated IRS-1 (Ser307) and TRB3 were significantly increased, while the levels of phosphorylated IRS1 (Tyr612) and phosphorylated Alt were decreased. Moreover, the expression of FGF21, FAS, SCD1, PPAR gamma and ADRP was significantly increased. The expression of ATF4, ATF5, eIF2a, GRP78, CHOP and phosphorylated level of PERK were also increased. The expression of FGF21 and TRB3 was significantly down-regulated, and the lipid droplets were notably reduced after the ER stress was inhibited by TUDCA. The expression of FGF21 was significantly decreased when the IRE1 pathway of the UPR was inhibited by STF-083010. Conclusions: Activation of Wnt/beta-catenin signaling pathway could cause insulin resistance and lipogenesis in hepatocytes via regulation of the IRE1 pathway of the ER stress and UPR, providing new targets for the treatment of metabolic disorders. (C) 2020 Elsevier Inc. All rights reserved.

Antidiabetic Potential of Green Seaweed Enteromorpha prolifera Flavonoids Regulating Insulin Signaling Pathway and Gut Microbiota in Type 2 Diabetic Mice

JOURNAL OF FOOD SCIENCE

Authors: Yan, Xin; Yang, Chengfeng; Lin, Guopeng; Chen, Yuqing; Miao, Song; Liu, Bin; Zhao, Chao

Abstract

This study aimed to investigate the antidiabetic activity of water-ethanol extract of green macroalgae Enteromorpha prolifera (EPW) and its flavonoid-rich fraction less than 3 kDa (EPW3) in type 2 diabetic mice induced by streptozotocin and a high-sucrose/high-fat diet. The major active compounds were identified using ultra-performance liquid chromatography coupled with quadrupole-time-of-flight-tandem mass spectrometry. Quantitative gene expression analysis of the insulin signaling pathway was performed. The effects of EPW3 on gut microflora in diabetic mice were analyzed by high-throughput 16S rRNA gene sequencing. The results showed EPW3 treatment decreased the fasting blood glucose, improved oral glucose tolerance, and protected against liver and kidney injury with reduced inflammation in diabetic mice. The active principle of EPW3 revealed hypoglycemic effect as indicated by activation of the IRS1/PI3K/AKT and inhibition of the JNK1/2 insulin pathway in liver. Furthermore, the treatment significantly enriched the abundance of Lachnospiraceae and Alisties, which were positive correlation of metabolic phenotypes. These findings indicated that EPW3 possessed great therapeutic potential as adjuvant therapy for type 2 diabetes.