Palladin is a novel binding partner of ILKAP in eukaryotic cells
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Authors: Zhou, Wang; Cui, Shusen; Han, Shuhai; Cheng, Baiqi; Zheng, Yu; Zhang, Yingjiu
Abstract
Palladin was a novel binding partner of ILKAP in eukaryotic cells. Palladin's C-terminal fragment including only its last three Ig domains (residues 710-1106) and the PP2C domain of ILKAP (residues 108-392) were necessary and sufficient for their interaction. The biological significance of the interaction between palladin and ILKAP was that palladin recruited the cytoplasmic ILKAP to initiate ILKAP-induced apoptosis. Our results suggested that palladin played a specific role in modulating the subcellular localization of the cytoplasmic ILKAP and promoting the ILKAP-induced apoptosis. (C) 2011 Elsevier Inc. All rights reserved.
CHST15 promotes the proliferation of TE-1 cells via multiple pathways in esophageal cancer
ONCOLOGY REPORTS
Authors: Wang, Xin; Cheng, Guowei; Zhang, Tao; Deng, Lei; Xu, Kunpeng; Xu, Xin; Wang, Wenqing; Zhou, Zongmei; Feng, Qinfu; Chen, Dongfu; Bi, Nan; Wang, Luhua
Abstract
Esophageal squamous cell carcinoma (ESCC) is a common type of esophageal cancer and is prevalent worldwide. Understanding the mechanisms underlying its formation and the search for more effective therapeutic strategies are critical due to the occurrence of chemotherapeutic drug resistance. The aim of the present study was to determine the functional relevance and therapeutic potential of carbohydrate sulfotransferase 15 (CHST15) in ESCC. CHST15 levels were measured in different ESCC cell lines and evaluated in ESCC tissues using tissue chip immunohistochemistry. Cell growth and apoptosis assays, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, and clonogenic assays were conducted using TE-1 cells and lenti-shCHST15 virus constructs were used to investigate the function of CHST15 in cell proliferation and apoptosis. mRNA microarray analysis was performed to determine the underlying mechanism of CHST15 regulation in TE-1 cell proliferation and apoptosis. The results showed that knockdown of CHST15 inhibited TE-1 cell growth and proliferation, but induced cell apoptosis. CHST15 was more frequently detected in ESCC tissue compared with that in normal esophageal tissue. Microarray data analysis indicated that the inhibition of cell proliferation and activation of cell apoptosis in CHST15-knockdown cells may be caused by altered CHST15/ILKAP/CCND1 and CHST15/RABL6/PMAIP1 signaling axes, respectively.
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