IL-17RA[Biotin]/IL-17A Inhibitor Screening Assay Kit

Intended Use

IL-17RA/IL-17A Inhibitor Screening Assay Kit is designed for screening inhibitors of IL-17A/IL-17RA interaction. The IL-17RA/IL-17A Inhibitor Screening Assay Kit comes in a convenient 96-well format, with biotin-labeled IL-17RA, purified IL-17A, streptavidinlabeled HRP, and assay buffer for 100 binding reactions.

Contents of Kit

1. IL-17RA[Biotin]: 2 μg, -80°C ;
2. IL-17A: 10 μg, -80°C;
3. 3x IL-17A assay buffer: 50 ml, -20°C;
4. Streptavidin-HRP: 15 μl, +4°C;
5. Blocking buffer: 50 ml, +4°C;
6. HRP chemiluminescent substrate A (translucent bottle): 6 ml, +4°C;
7. HRP chemiluminescent substrate B (brown bottle): 6 ml, +4°C;
8. White 96-well microplate: 1, +4°C .

Storage

One year from date of receipt when stored as directed.

General Description

IL-17A is a pro-inflammatory cytokine produced by TH17 cells that plays a key role in inflammation, autoimmunity, and host defense. It has been shown to stimulate the production of pro-inflammatory cytokines, namely IL-6 and IL-8, by synoviocytes. Due to its role as a mediator of inflammation, the IL-17 pathway has become a popular target for treating diseases that have a strong inflammatory component such as psoriasis, arthritis, Crohn's disease, and ankylosing spondylitis.