Human DFFA blocking peptide

Nature

Synthetic

Tag/Conjugate

Unconjugated

SequenceSimilarities

19 amino acids near the carboxy terminus of human DFF45.

Purity

> 95 % by SDS-PAGE.

Format

Lyophilized

Preservative

0.02% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. PBS with 0.1% BSA 0.02% sodium azide pH7.2

Reconstitution

DFFA peptide is supplied as 1mg of dried peptide. When the peptide is reconstituted in 1 mL strile water, the concentration will be 1 mg/mL.

Introduction

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Synonyms

DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; DFF1; ICAD; DFF-45; DNA fragmentation factor subunit alpha; DFF45; inhibitor of CAD; DNA fragmentation factor 45 kDa subunit;