Product Overview
A DNA sequence encoding the N-terminal segment (Met 1-Arg 342) of the influenza A H5N8 hemagglutinin (A/duck/NY/191255-59/2002 (H5N8)) (AAP72011.1), termed as HA1, was fused with a C-terminal polyhistidine tag.
Molecular Weight
The secreted recombinant HA1 subunit of influenza A H5N8 HA (A/duck/NY/191255-59/2002 (H5N8)) comprises 337 amino acids and has a predicted molecular mass of 38.2 kDa. As a result of glycosylation, it migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 97 % as determined by SDS-PAGE
Format
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H5N8 HA;H5N8 HA; IAV H5N8 HA
Citations
Publication ()
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COA
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