serum, plasma, other biological fluids
For the quantitative measurement of Hydroxyproline in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. This product is intended for research use only.
Contents of Kit
1. Anti-Hydroxyproline Microplate: 96 Wells (12 x 8 Well strips)
2. Hydroxyproline Lyophilized Standard: 2 x 4,000 nmol
3. Sample Diluent: 1 x 20 mL
4. 100X Hydroxyproline-Biotin Complex: 1 x 60 μL
5. 100X Avidin-HRP Conjugate: 1 x 120 μL
6. Biotin Complex Diluent: 1 x 10 mL
7. Conjugate Diluent: 1 x 10 mL
8. 25X Wash Buffer: 1 x 30 mL
2-8°C for 6 Months -20°C for 12 Months Avoid freeze thaw.
9. TMB Substrate: 1 x 10 mL
10. Stop Solution: 1 x 10 mL
2-8°C for 6 months.
Upon receipt store kit at 4°C for 6 months or -20°C for 12 months (with noted exceptions below). Avoid multiple freeze/thaw cycles.
Hydroxyproline is a major component of the protein collagen. Hydroxyproline and proline play key roles for collagen stability. They permit the sharp twisting of the collagen helix. Hydroxyproline is found in few proteins other than collagen. For this reason, hydroxyproline content has been used as an indicator to determine collagen and/or gelatin amount. Hydroxyproline is produced by hydroxylation of the amino acid proline by the enzyme prolyl hydroxylase following protein synthesis (as a post-translational modification). The enzyme catalysed reaction takes place in the lumen of the endoplasmic reticulum. Although it is not directly incorporated into proteins, hydroxyproline comprises roughly 4% of all amino acids found in animal tissue, more than seven amino acids which are directly incorporated.