Hyaluronidase Activity ELISA Kit

Sample

biological samples

Intended Use

96-well ELISA Assay for Detection and Quantification of Hyaluronidase Activity

Storage

Kit: store at -20°C
Pack: store Part 1 at -20°C and Part 2 at 4°C to -20°C

General Description

Hyaluronidases are a group of enzymes that degrade Hyaluronan (HA), a linear polysaccharide comprised of a repeating disaccharide of N-acetylglucosamine and D-glucuronic acid. HA is involved in many biological processes including structural support, cell migration and tissue turnover. Circulating HA levels have also been shown to correlate in several disorders such as liver disease and certain types of cancer. Hyaluronidases have become an important area of study due to their regulatory function in HA metabolism and their role in physiological processes such as fertilization and wound healing. Research has also shown that hyaluronidases are involved in several pathological processes such as bacterial pathogenesis, the spreading of toxins/venoms and cancer progression, making hyaluronidases a potential pharmacological target.

Standard Curve

HA standard curve was generated using non-linear regression analysis with GraphPad Software. A log[agonist] vs.response-variable slope (four parameter) analysis was utilized.

A sample titration using human serum with a 2 hour reaction time. Data interpolated against an HA standard curve and analyzed using non-linear regression analysis with GraphPad Software.