Human Schistosoma IgG ELISA Kit

Sample

Human sera

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For the qualitative determination of serum antibodies in humans, primarily IgG, to Schistosoma spp. using the ELISA technique

Contents of Kit

1. Microwell test strips containing Schistosoma SEA antigens - 96 test wells in a test strip holder.
2. Enzyme conjugate: One (1) bottle containing 11 ml of Protein A Peroxidase (HRP) in a stabilizing buffer with Thimerosal.
3. Positive control: One (1) vial containing 1 ml of diluted rabbit Schistosoma-positive sera in buffer with Thimerosal.
4. Negative control: One (1) vial containing 1 ml of diluted Schistosoma-negative human sera in buffer with Thimerosal.
5. TMB Substrate: One (1) bottle containing 11 ml of the (TMB).
6. Wash concentrate solution (20X): One (1) bottle containing 25 ml of concentrated buffer and surfactant with Thimerosal.
7. Dilution buffer: Two (2) bottles containing 30 ml of buffered protein solution with Thimerosal.
8. Stop solution: One (1) bottle containing 11 ml of 0.73 M phosphoric acid.

Storage

Reagents, strips and bottled components:
1. Store between 2°C - 8°C.
2. Squeeze bottle containing diluted wash buffer may be stored at room temperature.

Performance Characteristics

Positive Agreement: 100% (12/12)
Negative Agreement: 85% (34/40)
* Reference Method refers to a commercially available ELISA.