Intended Use
The described assay is intended for the quantitative determination of human pancreatic elastase in stool. For research use only.
Contents of Kit
1.PLATE Microtiter plate, pre-coated, 12 x 8 wells
2.WASHBUF ELISA wash buffer concentrate, 10 x, 2 x 100 ml
3.Extraction buffer concentrate, 2.5 x, 1 x 100 ml
4.CONJ Conjugate concentrate (mouse anti pancreatic elastase), 1 x 200 μl
5.STD Standard, lyophilized, 4 x 5 vials
6.CTRL1 Control, lyophilized, 4 x 1 vial
7.CTRL2 Control, lyophilized, 4 x 1 vial
8.SUB TMB substrate (Tetramethylbenzidine), ready to use, 1 x 15 ml
9.STOP ELISA stop solution, ready to use, 1 x 15 ml
Storage
Store the kit at 2-8°C.
Sensitivity
The LoB (limit of blank) was evaluated without considering sample dilution factor according to the guideline CLSI EP17-A2 and resulted in 0.66 μg/ml.
General Description
Pancreatic elastase is an anionic endoprotease of the serine protease family with a molecular weight of 26 kDa. Together with other digestive enzymes it is synthesized as an inactive pro-enzyme in the acinar cells of the pancreas and is secreted into the duodenum. After its activation, pancreas elastase cleaves peptides after neutral amino acids.
Pancreas elastase is mainly bound to bile salts during intestinal passage and is not degraded. In human feces it is 5–6 fold more concentrated than in pancreatic juice. The stool concentration reflects the secretory capacity of the pancreas.
Indications:
Diagnosis/exclusion of exocrine pancreas in sufficiency in case of unexplained diarrhea, constipation, steatorrhea, flatulence, weightloss, upper abdominal pain, and food into lerances.
Monitoring of exocrine pancreas function in cystic fibrosis, diabetes mellitus, or chronic pancreatitis.
Citations
Publication ()
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