Human Omentin-1 ELISA

Sample

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Human Omentin-1 ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human omentin-1.

Contents of Kit

Biotin Labelled Antibody Conc. (50×), concentrated, 0.28 ml
Streptavidin-HRP Conjugate, ready to use, 13 ml
Master Standard, lyophilized, 2 vials
Quality Control HIGH, lyophilized, 2 vials
Quality Control LOW, lyophilized, 2 vials
Biotin-Ab Diluent, ready to use, 13 ml
Dilution Buffer, ready to use, 20 ml
Wash Solution Conc. (10×), concentrated, 100 ml
Substrate Solution, ready to use, 13 ml
Stop Solution, ready to use, 13 ml

Storage

Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Performance Characteristics

It is intended for research use only.
The total assay time is less than 3.5 hours.
The kit measures omentin-1 in serum and plasma (EDTA, citrate, heparin).
Assay format is 96 wells.
Quality Controls are human serum based. No animal sera are used.
Standard is recombinant protein based.
Components of the kit are provided ready to use, concentrated or lyophilized.

Detection Range

2-64 ng/ml

Detection Limit

0.5 ng/ml

Sensitivity

Limit of Detection (LOD) (defined as concentration of analyte giving absorbance higher than mean absorbance of blank plus three standard deviations of the absorbance of blank: A_blank + 3×SD_blank) is calculated from the real human omentin-1 values in wells and is 0.5 ng/ml.
Note: Dilution Buffer is pipetted into blank wells.

General Description

Omentin (intelectin-1, intestinal lactoferin receptor, endothelial lectin HL-1, galactofuranosebinding lectin) is newly identified secretory protein that is highly and selectively expressed in visceral adipose tissue relative to subcutaneous adipose tissue (adipokine). The mature omentin is secretory glycoprotein consisting of 295 amino acids and 1-linked oligosacharides, and its basic structural unit is a 120-kDa homotrimer in which 40-kDa polypeptides are bridged by disulfide bonds. Omentin has been identified in other tissue at lower expression levels such are Paneth cells, endothelial cells, and visceral adipose stromal-vascular cells.
A homolog of omentin has been identified that shares 83% amino acid identity with omentin and was referred to as omentin 2. The two omentin genes, omentin 1 and omentin 2, are localized adjacent to each other in chromosomal region, which has been previously linked to type 2 diabetes in several populations.
To determine the impact of obesity-dependent insulin resistance on the regulation of two omentin isoforms, gene expression and plasma levels were measured in lean, overweight, and obese subjects. Omentin-1 was shown to be the major circulating isoform in human plasma. Lean subjects had significantly higher omentin-1 plasma levels than obese and overweight subjects. In addition, higher plasma omentin-1 levels were detected in women compared with men. Plasma omentin-1 levels were inversely correlated with BMI, waist circumference, leptin levels, and insulin resistance as measured by homeostasis model assessment and positively correlated with adiponectin and HDL levels. In summary, decreased omentin-1 levels are associated with increasing obesity and insulin resistance.
An independent experiment reported that the addition of recombinant omentin-1 in vitro did not affect basal glucose uptake but did enhance insulin-stimulated glucose uptake in both subcutaneous and omental human adipocytes. Omentin-1 increased Akt phosphorylation in the absence and presence of insulin and may regulate insulin action.
A recent study of women with the polycystic ovary syndrome (PCOS) found significantly reduced omentin-1 mRNA expression and protein levels in adipose tissue in overweight PCOS women. In addition, significantly lower plasma omentin-1 levels were detected in these women.

Standard Curve