Detection Method
Competitive ELISA
Intended Use
Enzyme immunoassay for the manual and automated measurement of normetanephrine in human urine.
Contents of Kit
1. Assay Buffer, Concentrate (10x): 1 x 50 mL
2. Acylation Reagent: 1 x 2 mL
3. Indicator Buffer: 1 x 10 mL
4. Hydrolyzation Tubes: 50 x
5. HCl: 1 x 20 mL
6. Microtiter Plate: 1 x 12 x 8
7. Standard A-G: 1 x 7 x 0.35 mL
8. Control 1±2: 1 x 2 x 0.5 mL
9. Normetanephrine Biotin, lyophilized: 3 x
10. Normetanephrine Antiserum: 1 x 7 mL
11. Enzyme Conjugate, Concentrate (100x): 1 x 250 μL
12. PNPP Substrate Tablets: 9 x
13. PNPP Substrate Buffer: 1 x 27 mL
14. PNPP Stop Solution: 1 x 15 mL
15. Wash Buffer, Concentrate (10x): 1 x 50 mL
16. Adhesive Foil: 3 x
Storage
Store components of the kit at 2-8°C or -20°C upon arrival up to the expiration date. For more detailed information, please download the following document on our website.
Citations
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Chan, ECY; Ho, PC; et al. Preparation and characterization of immunogens for antibody production against metanephrine and normetanephrine. JOURNAL OF IMMUNOLOGICAL METHODS 266:143-154(2002).
Lyte, M; Erickson, AK; et al. Norepinephrine-induced expression of the K99 pilus adhesin of enterotoxigenic Escherichia coli. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 232:682-686(1997).