Human, Mouse, Rat CARTPT/Cartpt ELISA Kit (DEIA3258)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell lysates, culture supernatant, plasma, serum
Species Reactivity
Human, Mouse, Rat
Intended Use
The CARTPT/Cartpt (Human, Mouse, Rat) ELISA Kit is an in quantitative assay for detecting CART peptide based on the principle of Competitive Enzyme Immunoassay.
Contents of Kit
1. CART Microplate
2. Wash Buffer Concentrate (20X)
3. Standard CART Peptide
4. Anti-CART polyclonal antibody
5. Assay Diluent A
6. Assay Diluent B
7. Biotinylated CART peptide
8. HRP-Streptavidin concentrate
9. Positive control
10. TMB One-Step Substrate Reagent
11. Stop Solution
Storage
The kit may be stored for up to 6 months at -20°C from the date of shipment. Standard, Biotinylated CART peptide, and positive control should be stored at -20°C or -80°C (recommended at -80°C) after arrival. Opened Microplate Wells and antibody may be stored for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Avoid multiple freeze-thaws for Standard, Biotinylated CART peptide and positive control.
Precision
Intra-assay: CV<10%
Inter-assay: CV<15%
Detection Range
1-100 ng/mL.
Sensitivity
1.35 ng/mL or 497.87 pM

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References


Folger, JK; Jimenez-Krassel, F; et al. Regulation of Granulosa Cell Cocaine and Amphetamine Regulated Transcript (CART) Binding and Effect of CART Signaling Inhibitor on Granulosa Cell Estradiol Production During Dominant Follicle Selection in Cattle. BIOLOGY OF REPRODUCTION 89:-(2013).
Hansell, NK; Agrawal, A; et al. Can We Identify Genes For Alcohol Consumption In Samples Ascertained For Heterogeneous Purposes?. ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH 33:729-739(2009).

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