The human LAYN ELISA kit is for the quantitative determination of human LAYN.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.
Contents of Kit
1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-LAYN monoclonal antibody.
3. Detection Antibody: 0.6 mg/mL of rabbit anti-LAYN polyclonal antibody conjugated to horseradish-peroxidase(HRP).
4. Standard: Each vial contains 65 ng of recombinant LAYN. Reconstitute with 1 mL detection antibody dilution buffer.
Detection Antibody should be proteced from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.
The minimum detectable dose of human LAYN was determined to be approximately 7.8125 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.