Human Interleukin 20 Receptor, Alpha, IL20RA ELISA Kit

Sample

Plasma, tissue homogenates and other biological fluids.

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The human IL20RA / IL20R1 ELISA Pair Set is for the quantitative determination of human IL20RA / IL20R1.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

Contents of Kit

Capture Antibody: 0.5 mg/mL of mouse anti-IL20RA monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating.
Detection Antibody: 0.5 mg/mL rabbit anti-IL20RA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody diluteion buffer before use.
Standard: Each vial contains 70ng of recombinant IL20RA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.

Storage

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. Do not freeze!
Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted standard at -80°C for up to 1 month. Avoid repeated freezethaw cycles.

Sensitivity

The minimum detectable dose of human IL20RA / IL20R1 was determined to be approximately 31.25 pg/ml.
This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

General Description

Interleukin-20 receptor subunit alpha also known as IL-20 receptor subunit alpha, IL-20R-alpha, Cytokine receptor family 2 member 8, IL20RA and IL-20R1 is a single-pass type I membrane protein which belongs to the type II cytokine receptor family. IL20RA / IL20R1 contains two fibronectin type-III domains. The interleukin-20-receptor I complex (IL-20-RI) is composed of two chains, IL20RA and IL20RB. IL20RA / IL20R1 forms heterodimer with IL20RB. Its ligands are the three members of the IL19 subfamily of cytokines, IL-19, IL-20 and IL-24. These cytokines are important in the manifestation of psoriatic lesions. All three are capable of signaling through IL-20RA / IL20RB complex. The ligand binding to receptor B creating a high-affinity binding site for the receptor A which is recruited to complete the complex. In addition, IL20RA / IL20R1 also forms a heterodimer with the unique and specific receptor IL10RB and functions as the receptor for IL26. IL20RA / IL20R1 is widely expressed with highest levels in skin, testis and brain. The expression of both IL20RA and IL20RB is found to be upregulated in psoriatic skin lesions on keratinocytes.

Standard Curve