Human IDO2 Inhibitor Screening Assay Kit

Species Reactivity

Human

Intended Use

The IDO2 Inhibitor Screening Assay Kit is designed to measure IDO2 enzyme inhibition. The kit comes in a convenient format with enough reaction solution and enzyme to perform a total of 100 reactions. The IDO2 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=320 – 325 nm.

Contents of Kit

1. IDO2 His-Tag: 400 μg, -80°C ;
2. IDO2 Reaction Solution: 2 x 10 ml, -80°C;
3. 1x IDO2 Assay Buffer: 1 ml, -80°C;
4. UV transparent 96-well plate: 1, Room Temp.

Storage

At least 6 months from date of receipt when stored as directed.

General Description

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.