Human Cardiotrophin-like Cytokine Factor 1 (CLCF1) ELISA Kit (DEIA6938)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
EDTA plasma, serum, cell culture supernatants
Species Reactivity
Human
Intended Use
Human CLCF1 immunoassay is a 3.5 - 4.5 hour solid phase ELISA designed to measure human CLCF1 in cell culture supernates, serum, and plasma.
Contents of Kit
1. CLCF1 Microplate
2. CLCF1 Standard
3. Detection Antibody Concentrate
4. Positive Control
5. Anti Rabbit IgG-HRP Conjugate
6. Dilution Buffer
7. Wash Buffer
8. TMB Substrate Solution
9. Stop Solution(0.5 M HCl)
10. Plate Sealer
Storage
Store at 2-8°C for up to 6 months. For longer storage, unopened Standard, Positive Control and Detection Antibody Concentrated should be stored at -20 or -70°C. Do not use past kit expiration date.
Precision
Intra-assay Precision: 4-6%
Inter-assay Precision: 8-12%
Detection Range
1.56-100 ng/mL
Sensitivity
50 pg/mL

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References


Burger, R; Bakker, F; et al. Functional significance of novel neurotrophin-1/B cell-stimulating factor-3 (cardiotrophin-like cytokine) for human myeloma cell growth and survival. BRITISH JOURNAL OF HAEMATOLOGY 123:869-878(2003).
Timmermann, A; Kuster, A; et al. A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor. EUROPEAN JOURNAL OF BIOCHEMISTRY 269:2716-2726(2002).

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