Human BALP(Bone Alkaline Phosphatase) ELISA Kit

Liver dysfunction is a major health problem worldwide. Stem cells therapy has opened up new avenues for researches to treat liver diseases due to their multi lineage differentiation. As mesenchymal stem cells (MSCs) can be differentiated into hepatic lineages in the presence of different exogenous factors, the current study aimed to investigate the impact of carbon tetrachloride (CCl4) induced liver injured mice serum on MSCs differentiation toward hepatocytes in vitro. Male Balb/c mice were treated for liver injury with CCl4 as determined through biochemical tests spectrophotometrically and different growth factors (EGF, HGF) quantification through Sandwich ELISA in both normal and CCl4-induced liver injured mice serum. Mice bone marrow derived-MSCs at second passage were treated with normal and CCl4-induced liver injured mice serum. After 7 days, serum treated MSCs were investigated for hepatocytes like characteristics through RT-PCR. Serum biochemical tests (Bilirubin, ALT and ALP) and sandwich ELISA results of EGF and HGF showed marked increase in CCl4 treated mice serum as compared to normal mice serum(.) Periodic acid Schiff's staining and urea assay kit confirmed high level of glycogen storage and urea production in cells treated with CCl4-induced liver injured mice serum. RT-PCR results of CCl4-induced liver injured mice serum treated cells also showed expression of hepatic markers (Albumin, Cyto-8, Cyto-18, and Cyto-19). This study confirmed that CCl4-induced liver injured serum treatment can differentiate MSCs into hepatocyte-like cells in vitro.

Herein, a photoelectrochemical (PEC) biosensor we proposed was used to detect let-7a via studying the photocurrent response change resulted from the change of the toehold-mediated strand displacement reaction (TSDR) between the AuNPs-H-2 (a conjugate of gold nanoparticles and DNA strand H2) and let-7a. Without the participation of let-7a, after the alkaline phosphatase (ALP) were immobilized at Bi2S3 surface through modified on the AuNPs-H2, ALP converted the ascorbic acid 2-phosphate (AAP) to produce ascorbic acid (AA), AA could be catalytically oxidized to provide electrons, resulting in the enhancement of photocurrent signal on the Bi-2 S-3. In contrast, the conjugate of ALP@AuNPs-H2 (a conjugate of ALP and AuNPs-H2) were compelled away from Bi2S3 surface when the let-7a participated in the process and hybridized with HI The fuel DNA assisted the recycling of the let-7a via another TSDR, the recovered let-7a could participate in the next enzyme-free cycle. The PEC biosensor not only exploited TSDR signal amplification strategy, but also achieved good linearity in the range of 0.01 nM-1000 nM with a low detection limit of 6.7 pM. Due to the excellent performance, we believe it will offer more opportunities for bioanalysis and clinical biomedicine.