Human Anti-Phosphatidyl Serine IgG / IgM ELISA Kit

Sample

serum, plasma

Species Reactivity

Human

Detection Method

Indirect ELISA

Intended Use

Anti-Phosphatidyl Serine is an indirect solid phase enzyme immunoassay ( ELISA ) for measurement of IgG and IgM class autoantibodies against phosphatidyl serine in human serum or plasma.

Contents of Kit

1. Qty.1: Divisible microplate consisting of 12 modules of 8 wells each, coated with highly purified Phosphatidyl serine and
saturated with β-2-Glycoprotein I. Ready to use.
2. 6 vials, 1.5 mL each combined Calibrators with IgG and IgM class Anti-Phospholipid antibodies ( A-F ) in a serum/buffer
matrix ( PBS, BSA, NaN3 <0, 1% ( w/w ) ) containing: IgG: 0; 6.3; 12.5; 25; 50; and 100 GPL U/mL and IgM: 0; 6.3; 12.5; 25; 50;
100 MPL U/mL. Ready to use.
3. 2 vials, 1, 5 mL each Anti-Phospholipid Controls in a serum/buffer matrix ( PBS, BSA, NaN3 <0, 1% ( w/w ) ) positive and
negative, for the respective concentrations see the enclosed QC Certificate. Ready to use.
4. 1 vial, 20 mL Sample buffer ( Tris, NaN3 <0.1% ( w/w ) ), yellow, concentrate ( 5x ).
5. 1 vial, 15 mL Enzyme conjugate solution ( PBS, Proclin 300 <0.5% ( v/v ) ), ( light red ) containing polyclonal rabbit antihuman
IgG; labelled with horseradish peroxidase. Ready to use.
6. 1 vial, 15 mL Enzyme conjugate solution ( PBS, Proclin 300 <0.5% ( v/v ) ), ( light red ) containing polyclonal rabbit antihuman
IgM; labelled with horseradish peroxidase. Ready to use.
7. 1 vial, 15 mL TMB substrate solution. Ready to use.
8. 1 vial, 15 mL Stop solution ( contains acid ). Ready to use.
9. 1 vial, 20 mL Wash solution ( PBS, NaN3 <0.1% ( w/w ) ), concentrate ( 50x ).

Storage

The kit should be stored at 2-8°C. For more detailed information, please download the following document on our website.