Intended Use
In vitro quantitative determination of Hepc25 concentrations in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
1. ELISA Microplate (Dismountable): 8×12, 2-8°C/-20°C. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
2. Lyophilized Standard: 2 vials, 2-8°C/-20°C. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Sample Dilution Buffer: 20 ml, 2-8°C
4. Biotin-labeled Antibody (Concentrated): 120 μl, 2-8°C (Avoid Direct Light)
5. Antibody Dilution Buffer: 10 ml, 2-8°C
6. HRP-Streptavidin Conjugate (SABC, 100×): 120 μl, 2-8°C (Avoid Direct Light)
7. SABC Dilution Buffer: 10 ml, 2-8°C
8. TMB Substrate: 10 ml, 2-8°C (Avoid Direct Light)
9. Stop Solution: 10 ml, 2-8°C
10. Wash Buffer (25X): 30 ml, 2-8°C
11. Plate Sealer: 5 pieces
12. Product Description: 1 copy
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development,and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference,since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
