Recombinant HIV type 1 gp140 (A244) (a.a. 44-681) [His]

Name: Recombinant HIV type 1 gp140 (A244) (a.a. 44-681) [His]
SKU: DAG2132
Rating: 5 (1 reviews)

Recombinant HIV type 1 gp140 (A244) (a.a. 44-681) from HEK293 cells [His]

COA

SDS

Datasheet

Summary

Product Overview

C-terminal 6xHis tagged gp140 (HIV-1/Clade AE)(A244) protein (a.a. 44-681). The same as the Thai trial (RV144) strain. One a.a. difference from 90TH_CM244

Nature

Recombinant

Tag/Conjugate

His

Alternative Names

Human Immunodeficiency Virus; HIV gp140; HIV; HIV-1 gp140; HIV-1; HIV type 1 gp140

Purity

≥ 95%

Format

Liquid

Concentration

1 mg/ml

Buffer

PBS

Preservative

None

Storage

Stable for a month at 4°C. For long term storage, split it into small aliquots and keep at -80°C. Avoid repeated freezing and thawing.

Introduction

The HIV-1 envelope (Env) glycoproteins play an essential role in the virus replication cycle by mediating the fusion between viral and cellular membranes during the entry process. The Env glycoproteins are synthesized as a polyprotein precursor, gp160, that is cleaved by cellular proteases to the mature surface glycoprotein gp120 and the transmembrane glycoprotein gp41. During virus assembly the gp120/gp41 complex is incorporated as heterotrimeric spikes into the lipid bilayer of nascent virions.

Keywords

Human Immunodeficiency Virus; HIV gp140; HIV; HIV-1 gp140; HIV-1; HIV type 1 gp140

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

A facile synthesis of 6-[(4-nitrophenyl)thio]-substituted 2-methylene-2,3-dihydro-1,4-oxazepines from N-propargylic beta-enaminones

SYNTHETIC COMMUNICATIONS

Authors: Kelgokmen, Yilmaz; Korkmaz, Esra; Zora, Metin

Abstract

A one-pot process for the synthesis of 6-[(4-nitrophenyl)thio]-substituted 2-methylene-2,3-dihydro-1,4-oxazepines is reported. When reacted with 4-nitrobenzenesulfenyl chloride, N-propargylic beta-enaminones afforded alpha-sulfenylated N-propargylic beta-enaminones, which, in the presence of zinc chloride, underwent electrophilic cyclization to yield 6-[(4-nitrophenyl)thio]-substituted 2-methylene-2,3-dihydro-1,4-oxazepines. Process was found to be general for various N-propargylic beta-enaminones along with large substrate scope and high functional group tolerance. This operationally easy method may provide quick access to a library of functionalized 1,4-oxazepines of pharmacological interest.

Overview of the Nucleic-Acid Binding Properties of the HIV-1 Nucleocapsid Protein in Its Different Maturation States

VIRUSES-BASEL

Authors: Mouhand, Assia; Pasi, Marco; Catala, Marjorie; Zargarian, Loussine; Belfetmi, Anissa; Barraud, Pierre; Mauffret, Olivier; Tisne, Carine

Abstract

HIV-1 Gag polyprotein orchestrates the assembly of viral particles. Its C-terminus consists of the nucleocapsid (NC) domain that interacts with nucleic acids, and p1 and p6, two unstructured regions, p6 containing the motifs to bind ALIX, the cellular ESCRT factor TSG101 and the viral protein Vpr. The processing of Gag by the viral protease subsequently liberates NCp15 (NC-p1-p6), NCp9 (NC-p1) and NCp7, NCp7 displaying the optimal chaperone activity of nucleic acids. This review focuses on the nucleic acid binding properties of the NC domain in the different maturation states during the HIV-1 viral cycle.