HDAC5 (Phospho-Ser259) ELISA Kit (DEIA-XYA782)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
2 x 96T
Sample
cultured cells
Species Reactivity
Human, Mouse
Intended Use
The HDAC5 (Phospho-Ser259) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HDAC5 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated HDAC5 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HDAC5 phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL
3. Quenching Buffer: 24 mL
4. Blocking Buffer: 50 mL
5. 10x Wash Buffer: 50 mL
6. 100x Anti-HDAC5 (Phospho-Ser259) Antibody (Rabbit Polyclonal): 60 μL, red
7. 100x Anti-HDAC5 Antibody (Rabbit Polyclonal): 60 μL, purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL, green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL, glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL, glass
11. Primary Antibody Diluent: 12 mL
12. Ready-to-Use Substrate: 12 mL
13. Stop Solution: 12 mL
14. Crystal Violet Solution: 12 mL
15. SDS Solution: 24 mL
16. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months

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References


Huynh, QK; et al. Evidence for the phosphorylation of serine(259) of histone deacetylase 5 by protein kinase C delta. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 506:173-180(2011).
Ishikawa, S; Nagai, Y; et al. The role of oxysterol binding protein-related protein 5 in pancreatic cancer. CANCER SCIENCE 101:898-905(2010).

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