Anti-Glycoprotein GPIIIa/GPIIb Complex monoclonal antibody, clone BQ-3

Background: Pancreatic autoantibodies (PAB) targeting GP2 and CUZD1 are Crohn's disease (CrD)-markers. The clinical significance of anti-GP2 antibodies has been assessed, but that of anti-CUZD1 remains elusive. The aim of the study was to assess the clinical utility of anti-CUZD1/anti-GP2 by novel cell-based indirect immunofluorescence (IIF) assays in CrD. Methods: A total of 212 CrD and 249 UC patients followed up at a London IBD centre were investigated to simultaneously detect PABs, anti-GP2 and anti-CUZD1 by IIF using primate pancreatic tissue, and HEK293 over-expressing CUZD1 or GP2. Results: Overall, 88 (41.5%) CrDs compared to 26 (10.4%) UCs (p < 0.001) tested positive for IgA and/or IgG anti-GP2 and/or anti-CUZD1 antibodies, while ASCA were found in 67.5% CrDs versus 19.2% UCs (p < 0.0001); ASCA and/or PAB (anti-GP2 or anti-CUZD1) were detected in 76% CrD versus 34% UC patients. IgG anti-GP2 antibodies were less prevalent in L2 phenotype (p = 0.002) and more prevalent in patients with stricturing disease (p = 0.0418), even when a higher cut-off (= 1000 RU) was used (p = 0.0396). Also, anti-GP2 IgG positive CrD patients had younger age of disease onset. IgA and/or IgG ASCA and anti-GP2 IgG antibody positive CrDs had younger onset of disease (p < 0.0001), were more likely to have both ileal and colonic disease (p < 0.0001) and had more stricturing (p < 0.0001) than seronegative patients. Clinical correlates were not found for anti-CUZD1 positivity. Conclusions: PAB testing increases ASCA's serological sensitivity for CrD. Anti-GP2 detection, in isolation or in combination with ASCA, stratify CrD patients who phenotypically are characterised by a much younger onset of disease, extensive and stricturing behaviour.

On the basis of the analysis of the sensor's working principle, a magnetostrictive displacement sensor was developed. The system which uses the AVR microcontroller as the control core measures time intervals through the TDC-GP2 timing chip and uses a serial port to receive commands and transmit data with PC. A circuit board is produced after the design. And The sensor was tested after installation and commissioning. The experiments show that this design improves sensor accuracy.