GLI4 Knockout Cell Lysate from 293T cell line for anti-GLI4 antibody specificity validation.
293T cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The GLI4 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
GLI4; GLI family zinc finger 4; GLI Kruppel family member GLI4; glioma associated oncogene family zinc finger 4; zinc finger protein GLI4; HKR4
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
The protein concentration was determined with BCA assay.
Store at -20°C. Avoid repeated freeze-thaw cycles.