GITRL/GITR (Biotiny) Inhibitor Screening Assay Kit

Intended Use

The GITR/GITRL Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of GITR/GITRL signaling.

Contents of Kit

1. GITR, Biotin-labeled: 2 μg, -80°C;
2. GITRL-His: 10 μg, -80°C;
3. Streptavidin-HRP: 15 μl, +4°C;
4. 3x GITR Assay Buffer: 50 ml, -20°C;
5. Blocking Buffer: 50 ml, +4°C;
6. HRP chemiluminescent substrate A (transparent bottle): 6 ml, +4°C;
7. HRP chemiluminescent substrate B (brown bottle): 6 ml, +4°C;
8. White 96-well microplate 1 +4°C.

Storage

One year from date of receipt when stored as directed.

General Description

Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR's natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno oncology therapies.