The Gab2 (Phospho-Tyr643) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Gab2 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Gab2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Gab2 phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates 2. 10x TBS: 24 mL 3. Quenching Buffer: 24 mL 4. Blocking Buffer: 50 mL 5. 10x Wash Buffer: 50 mL 6. 100x Anti-Gab2 (Phospho-Tyr643) Antibody (Rabbit Polyclonal): 60 μL, red 7. 100x Anti-Gab2 Antibody (Rabbit Polyclonal): 60 μL, purple 8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL, green 9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL, glass 10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL, glass 11. Primary Antibody Diluent: 12 mL 12. Ready-to-Use Substrate: 12 mL 13. Stop Solution: 12 mL 14. Crystal Violet Solution: 12 mL 15. SDS Solution: 24 mL 16. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months
Citations
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References
Anti-allergic and anti-inflammatory activity of Phellinus linteus grown on Panax ginseng
Panax ginseng (PG) or Phellinus linteus (PL) have been widely used as traditional medicine owing to their many biological activities, including anti-inflammatory and anti-allergic activities. Previously, our group produced PL that was grown on PG media (PGP) to enhance anti-cancer activities of PGP. Here we studied the anti-allergic activity of PGP and its mechanism of action. The ethyl acetate fraction of PGP exhibited the anti-allergic activity by suppressing beta-hexosaminidase release, a marker of degranulation, from antigen/immunoglobulin E (IgE)-stimulated RBL-2H3 cells. Exposure to PGP inhibited the level of antigen/IgE-induced TNF-alpha in RBL-2H3 cells. It markedly suppressed the phosphorylation of spleen associated tyrosine kinase, GRB2-associated-binding protein 2 (Gab2) and extracellular signal-regulated kinases proteins, which are required for the degranulation and production of pro-inflammatory cytokines. Its anti-inflammatory activity was observed in lipopolysaccharide-stimulated RAW 264.7 cells. In addition, PGP contained higher contents of Rg1 than PG. Our findings suggest that PGP might be developed as a therapeutic agent for IgE-mediated allergic diseases.
MicroRNA-218 Inhibits Tumor Angiogenesis of Human Renal Cell Carcinoma by Targeting GAB2
COA
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