FREE soluble RANKL High Sensitivity ELISA kit

Sample

serum, plasma

Species Reactivity

Human

Intended Use

3rd Generation ENZYME IMMUNOASSAY FOR THE QUANTITATIVE DETERMINATION OF FREE, SOLUBLE, UNCOMPLEXED HUMAN RANKL IN SERUM OR HEPARIN PLASMA.

Storage

Store the kit at 4°C upon receipt. For more detailed information, please download the following document on our website.

Performance Characteristics

Precision

Intra-assay: 2 samples of known concentrations weretested 5 times within one kit lot by one operator.
Inter-assay: 2 samples of known concentrations weretested 12 times within 3 different kit lots and by3 different operators.

General Description

RANKL, the receptor activator of nuclear factor kappa B ligand, a member of the tumor necrosis factor (TNF) family, is the main stimulatory factor for the formation ofmature osteoclasts and is essential for their survival. RANKL activates its specific receptor RANK, located on osteoclasts and dendritic cells. The effects are counteracted by OPG which acts as an endogenous soluble receptor antagonist.
The major source of RANKL are osteocytes, former osteoblasts that become embedded within the mineralized bone matrix. RANKL is a ~35 kD type II transmembrane-type protein and is cleaved to release a soluble biologically active product that forms a homotrimer.
RANKL and its specific receptor RANK are not only key regulators of bone remodeling but also play an essential role in immunobiology, e.g. lymph node formation, establishment of the thymic microenviroment, mammarygland development during pregnancy, bone metastasis in cancer and sex-hormone, progestin-driven breast cancer, thermoregulation, and finally in the development of type 2 diabetes mellitus.

Possible Indications:
1) Postmenopausal and senile osteoporosis
2) Glucocorticoid induced osteoporosis
3) Disease with locally increased resorption activity
4) Arthritis
5) Oncology
6) Type 2 diabetes mellitus

Reconstitution And Storage

STD (Standards) and CTRL (Controls): Pipette 700 μL of distilled or deionised water into each vial. Leave at room temperature (18-26°C) for 15 min. Reconstituted STD and CTRL are stable at -25°C or lower until expiry date stated on label. STDs and CTRLs are stable for3 freeze-thaw cycles.