Sample
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Detection Method
Competitive ELISA
Intended Use
Rat Fibrillin 1 ELISA Kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the Fibrillin 1. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.
Contents of Kit
1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL
Storage
All components of this kit are stable at 2-8°C until the kit's expiration date.
Detection Range
5-100 ng/mL
Citations
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McNearney, TA; Reveille, JD; et al. Pulmonary involvement in systemic sclerosis: Associations with genetic, serologic, sociodemographic, and behavioral factors. ARTHRITIS & RHEUMATISM-ARTHRITIS CARE & RESEARCH 57:318-326(2007).
Ono, RN; Sengle, G; et al. Latent Transforming Growth Factor beta-binding Proteins and Fibulins Compete for Fibrillin-1 and Exhibit Exquisite Specificities in Binding Sites. JOURNAL OF BIOLOGICAL CHEMISTRY 284:16872-16881(2009).