Epitestosterone [HRP]

Specificity

Each conjugate comprises antigen covalently bound to horseradish peroxide and is suitable as a tracer in immunoassay development.

Nature

Synthetic

Tag/Conjugate

HRP

Alternative Names

Epitestosterone; 17-hydroxy-10,13-dimethyl-1,2,6,7,8,9,11,12,14,15,16,17- dodecahydrocyclopenta[a]phenanthren-3-one

Format

The conjugate is supplied as a concentrate. Dilute as required and use working strength conjugate immediately after dilution

Preservative

None

Storage

2 - 8°C for up to 3 months / -20°C for long term storage

Introduction

Epitestosterone is an endogenous antiandrogen steroid, an epimer of the hormone testosterone. It is a weak competitive antagonist of the androgen receptor (AR) and a potent 5α-reductase inhibitor. Structurally, it differs from testosterone only in the configuration at the OH-bearing carbon, C17. Epitestosterone is believed to form in a similar way to testosterone; a 1993 study found that around 50% of epitestosterone production in human males can be ascribed to the testis, although the exact pathway of its formation is still the subject of research. It has been shown to accumulate in mammary cyst fluid and in the prostate. Epitestosterone levels are typically highest in young males; however, by adulthood, most healthy males exhibit a testosterone to epitestosterone ratio (T/E ratio) of about 1:1.