Human DNM3 blocking peptide

Well-differentiated/de-differentiated liposarcomas (WDLS/DDLS) encompass an intriguing disease model in which a temporal intersection occurs between the malignant transformation of mesenchymal cells and the process of adipogenesis. Deciphering the molecular events that trigger and are characteristic of the intersection of these oncogenic and normal processes is critical to affect the often morbid and lethal consequences of malignant tumors of fat. High-resolution genome-wide oligonucleotide array-based comparative genomic hybridization (aCGH) with matched gene expression analyses was performed on seven lipomas, one hibernoma, and 38 WD and DDLS to define and compare the genomic events associated with these tumors. WD and DDLS had complex karyotypes. On average, WDLS had 11.1 and DDLS had 22.7 chromosomal copy number aberrations. All of the liposarcomas had 12q13-q15 amplifications with varying peaks at CDK4 (12q14.1), HMGA2 (12q14.3), and MDM2 (12q15); 24% of the DDLS and no WDLS had 1p32.2 (JUN) amplifications; 33% WDLS and 35% DDLS had 1q24.3 amplifications involving DNM3 and miR-214/miR-199a2; 24% of the liposarcomas had 6q23-q24 amplifications (including MAP3K5). Amplifications in GLI1 (12q13.3), JUN, and MAP3K5 (6q23.3) were mutually exclusive and occurred predominately in the DDLS. 6q amplifications occurred primarily in retroperitoneal tumors and females represented the majority of those patients who developed fatty tumors prior to the age of 50 years old. This detailed genetic mapping provides insight into the heterogeneity of WD and DDLS and the chromosomal and genetic abnormalities that are present in and distinguish these mesenchymal malignancies. (C) 2010 Wiley-Liss, Inc.

Over the past years there has been a dramatic increase in the regulatory requirements for low emissions from both new and existing utility boilers and gas turbines. Traditional methods of reducing NOx emissions, such as: modification of the firing system; and/or post combustion treatment of the flue gas to remove NOx; are very expensive. One of the attractive alternative fuels for combustion in the utility boilers and stationary gas turbines may be methanol. Using methanol has become an important solution for emissions compliance due to their unique constituents and combustion characteristics. Methanol may be referred to as enviro fuel. The clean burning characteristics of methanol are expected to lead to clean pressure parts, turbine blades and lower maintenance than with fuel oil. Here, we present results of the Israel Electric Corporation (IEC) for specific 140 MWe unit consisting of tangential fired pressurized boiler designed by Combustion Engineering Inc by using the co-firing of methanol with heavy fuel oil and FT4C TWIN PAC 50 MWe GT designed by Pratt & Whitney by using the full methanol firing. The experiments performed for gas turbine tested different GT loads during methanol and LFO firing. The results presented here clearly show that with minor low cost fuel system retrofit methanol firing leads to significant NOx, SO2 and particulates emission reduction. NOx emissions were reduced more than 75% and are equal 75 mg/dNm3 at 15%O2. SO2 emissions were reduced to zero with methanol firing. Particulate emissions vary from 1.3 to 1.6 mg/dNm3 at 15% O2 with methanol firing, while with LFO this parameter was 13-37 mg/dNm3 at 15% O2. The experiments performed for the boiler tested different methanol fractions of the total boiler heat capacity (from 33% to 50% heat), at different boiler loads. The results presented here show that NOx, SO2 and particulate emissions were reduced more than 20%, 35% and 40% accordingly. We believe that the conclusions of the present work are general and can be applied to other boilers and gas turbines as well.