Human DNMBP ELISA Matched Antibody Pair

L-arginine has been demonstrated to stimulate cell proliferation and prevent LPS-induced death of intestinal cell. To identify genes responsed to arginine treatment in intestinal cell, gene expression profiles were analyzed using high-density oligonucleotide microarray in porcine intestinal epithelial cells (IPEC-1) treated with 100 (control) or 350 mu M arginine. Results showed that expression levels of 306 genes were up-regulated and 280 genes down-regulated in IPEC-1 cultured in medium containing 350 mu M arginine compared with that of the control. Although the biochemical functions of some genes had not previously been defined, we were able to identify some up-regulated genes that are known to play important roles in transportion (e.g. ATP6V1G2, SLC9A4), metabolism (e.g. AKRIC4, GHRL). transcription and signal transduction (e.g. PTGFR, OCT2), signaling and proliferation (e.g. UPTI, FGFR1). cell cycle and apoptosis (e.g. DRAM1. GADD45A), cytoskeletal and cell adhesion (e.g. DNMBP, ITGB2) and immune response (e.g. OAS1, IL15), and some down-regulated genes that are related to transportion and metabolism (e.g. SLC25A25, COX I). cytoskeletal and cell adhesion (e.g. PROC, COL1OA1) and immune response (e.g. SLA-DMA, CD80). The RT-PCR analysis demonstrated that 350 mu M arginine treatment increased ITGB (109%), AKRIC4 (367%), ATP6VIG2 (196%), DRAMI (88%), GHRL (243%) and SLC9A2 (138%). which were almost consistent with the data obtained from the microarray analysis. Our findings provided a molecular mechanism to explain cytoprotective effects of arginine and laid the foundation for future research on cellular mechanisms of arginine regulating cell growth and proliferation.

Genetic association of the dynamin binding protein gene (DNMBP) on chromosome 10 with late-onset Alzheimer's disease (AD) was reported among Japanese. Here, we assessed the genetic role of DNMBP in an extended Belgian AD group using a gene-wide association approach. A total of 18 SNPs across the DNMBP locus were genotyped in 555 late-onset AD patients and 638 healthy control individuals. Significant associations were observed for two SNPs (rs3740057 and rs10883421). Haplotype analysis identified association with haplotype blocks in the 3' region of DNMBP comprising rs2862919, rs11190302, rs10509739, rs2256700 and comprising rs3740057 and rs6584331. Stratification for APOE 0 status showed that association was only present in the APOE epsilon 4 negative subgroup. Sliding-window analyses provided further evidence for association with the 3'-end of DNMBP both for the total and for the APOE epsilon 4 negative group. Taken together our findings underscore a role for DNMBP in the genetic risk for late-onset AD in the Belgian Population (C) 2008 Elsevier Inc. All rights reserved.