Human Dimethyl Phthalate (DMP) ELISA Kit

Name: Human Dimethyl Phthalate (DMP) ELISA Kit
SKU: DEIANJ02
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Cell culture fluid, body fluid, tissue homogenate, serum or plasma

Species Reactivity

Human

Detection Method

cELISA

Intended Use

The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an enzyme-linked immunosorbent assay for the quantitative measurement of samples in serum, plasma, cell culture supernatants and urine.

Storage

Store all reagents at 2-8°C

Sensitivity

The sensitivity of this assay should be approximately 1.0ng/ml.

Standard Curve

Citations

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Immuno-PCR in the Analysis of Food Contaminants

Radomirović M, Gligorijević N, Rajković A

Int J Mol Sci2025 MarPubMed ID: 40243808Read Article

Applications: ELISA
Reactive species: Human
"Abstract: Food safety is a significant issue of global concern. Consumer safety and government regulations drive the need for the accurate analysis of food contaminants, residues and other chemical constituents of concern. Traditional methods for the detection of food contaminants often present challenges, including lengthy processing times and food matrix interference; they often require expensive equipment, skilled personnel or have limitations in sensitivity or specificity. Developing novel analytical methods that are sensitive, specific, accurate and rapid is therefore crucial for ensuring food safety and the protection of consumers. The immuno-polymerase chain reaction (IPCR) method offers a promising solution in the analysis of food contaminants by combining the specificity of conventional immunological methods with the exponential sensitivity of PCR amplification. This review evaluates the current state of IPCR methods, describes a variety of existing IPCR formats and explores their application in the analysis of food contaminants, including pathogenic bacteria and their toxins, viruses, mycotoxins, allergens, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, phthalic acid esters, pesticides, antibiotics and other food contaminants. Depending on the type of analyte, either sandwich or competitive format IPCR methods are predominantly used. This review also examines limitations of current IPCR methods and explores potential advancements for future implementation in the field of food safety."
Article snippet: The immuno-PCR method had a limit of detection of 1.98 pg/mL, with a linearity range spanning from 10 to 100 ng/mL. In comparison, a commercially available ELISA kit by CD Creative Diagnostics (NY, USA) for the detection of DMP in cell culture fluid, body fluids, tissue homogenates, serum or plasma has a sensitivity of approximately 1 ng/mL.