Rabbit Anti-Human DHCR7 (a.a. 351-450) Polyclonal antibody

Name: Rabbit Anti-Human DHCR7 (a.a. 351-450) Polyclonal antibody
SKU: CABT-BL4505
Rating: 5 (1 reviews)

Rabbit Anti-Human DHCR7 (a.a. 351-450) Polyclonal antibody for WB

COA

SDS

Datasheet

Key features and details

Target: DHCR7
Host: Rabbit
Application: WB
Species Reactivity: Mouse, Human, Rat, Chicken, Dog, Cow, Horse

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Mouse; Human; Rat; Chicken; Dog; Cow; Horse

Immunogen

KLH conjugated synthetic peptide derived from human DHCR7: 351-450/475

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-2000

General Notes

This product (Catalog No. CABT-BL4505) is a rabbit-derived IgG polyclonal antibody raised against human DHCR7, targeting the amino acid region 351–450 of the protein. This antibody exhibits cross-reactivity with DHCR7 from mouse, rat, chicken, dog, cow, and horse. It has been affinity purified by protein A. This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.
CABT-BL4505 is suitable for use in Western blot (WB) (1:500–1:2,000) application.
CABT-BL4505 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

7 dehydrocholesterol reductase; 7 DHC reductase; Delta 7 dehydrocholesterol reductase; Putative sterol reductase SR 2; Sterol Delta; DHCR7_HUMAN

Entrez Gene ID

1717

UniProt ID

Q9UBM7

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Single Nucleotide Polymorphisms of Vitamin D Metabolism-Related Gene DHCR7 Are Associated with Vascular Risk

DIABETES

Authors: Dalan, Rinkoo; Liew, Huiling; Goh, Liuh Ling; Mohamad, Safiyya Ali; Chew, Daniel E. K.; Boehm, Bernhard O.

Abstract

Varying relationship between 25-hydroxy-vitamin D, high density lipoprotein cholesterol, and serum 7-dehydrocholesterol reductase with sunlight exposure

JOURNAL OF CLINICAL LIPIDOLOGY

Authors: Patwardhan, Vivek G.; Khadilkar, Anuradha V.; Chiplonkar, Shashi A.; Mughal, Zulf M.; Khadilkar, Vaman V.

Abstract

BACKGROUND: Cholesterol and cholecalciferol are synthesized from a common substrate 7-dehydrocholesterol. 7-dehydrocholesterol is converted to cholesterol by 7-dehydrocholesterol reductase enzyme (DHCR7) and to cholecalciferol by ultraviolet B radiation from sunlight. OBJECTIVE: To examine the effect of sunlight exposure and serum DHCR7 levels on cholecalciferol and cholesterol levels and studying any interrelationship. METHODS: In a cross-sectional observational study, 307 apparently healthy men (aged 40-60 years) were assessed for sunlight exposure, lipid levels, serum DHCR7, 25 hydroxyvitamin D (25(OH)D), body composition, and dietary milk calcium intake. RESULTS: Vitamin D deficiency (25(OH)D <20 ng/mL, 1 ng/mL = 2.496 nmols/L) was found in 56% of subjects. Serum 25(OH)D concentrations increased significantly with increasing duration of sunlight exposure (P < .05). At lower sunlight exposure (<1 h/d), serum 25(OH)D levels were positively associated with high-density lipoprotein cholesterol (HDL-C) levels (P < .05) but at moderate sunlight exposure (1-2 h/d), there was no significant association. However, with higher sunlight exposure (>2 hid), serum 25(OH)D concentrations were significantly negatively associated with HDL-C (P < .05). At moderate and higher sunlight exposure, an inverse significant relationship was observed between 25(OH)D and serum DHCR7 (P < .05); however, at lower sunlight exposure, no significant relationship was seen. CONCLUSIONS: 25(OH)D seems to show a varying relationship with HDL-C and serum DHCR7 at different durations of sunlight exposure. (C) 2015 National Lipid Association. All rights reserved.