Human DDX1 peptide

Name: Human DDX1 peptide
SKU: DAG-P1500
Rating: 5 (1 reviews)

Synthetic Human DDX1 peptide

COA

SDS

Datasheet

Summary

Specificity

Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.

Nature

Synthetic

Tag/Conjugate

Unconjugated

SequenceSimilarities

Belongs to the DEAD box helicase family. DDX1 subfamily.Contains 1 B30.2/SPRY domain.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.

Alternative Names

The helicase domain is involved in the stimulation of RELA transcriptional activity.

Format

Liquid

Preservative

None

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Information available upon request.

Entrez Gene ID

1653

Antigen Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008]

Function

ATP binding; ATP-dependent helicase activity; DNA binding; DNA/RNA helicase activity; RNA helicase activity; chromatin binding; double-stranded RNA binding; exonuclease activity; nuclease activity; poly(A) RNA binding; poly(A) binding; protein binding; tr

Synonyms

DDX1; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DBP-RB; UKVH5d; ATP-dependent RNA helicase DDX1; DEAD box-1; DEAD box protein 1; DEAD box polypeptide 1; DEAD box protein retinoblastoma; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1;

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Aberrant copy numbers of ALK gene is a frequent genetic alteration in neuroblastomas

HUMAN PATHOLOGY

Authors: Subramaniam, Manish Mani; Piqueras, Marta; Navarro, Samuel; Noguera, Rosa

Abstract

A total of 50 neuroblastomas were assessed for frequency of ALK gene copy number aberrations by interphase fluorescence in situ hybridization using a break-apart fluorescence in situ hybridization probe. The data were compared with status of MYCN, 11q, 17q, and 1p36. We observed ALK aberrations (amplification, 1 of 45; gain, 15 of 45 and loss/imbalance, 11 of 45) in a total of 27 (60%) of 45 neuroblastomas. Synchronic MYCN and ALK aberrations accounted for 23 of 45 (51%) tumors: however, MYCN alterations were also detected in 11 (60%) of 18 tumors without ALK aberrations. Our data Suggest that copy number aberrations of the ALK gene is a frequent genetic event in the development of neuroblastomas. In addition, no correlation was observed between ALK aberrations and alterations of 11q, 17q, and 1p36. (C) 2009 Elsevier Inc. All rights reserved.

miRNAmotif-A Tool for the Prediction of Pre-miRNA-Protein Interactions

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Authors: Urbanek-Trzeciak, Martyna O.; Jaworska, Edyta; Krzyzosiak, Wlodzimierz J.

Abstract

MicroRNAs (miRNAs) are short, non-coding post-transcriptional gene regulators. In mammalian cells, mature miRNAs are produced from primary precursors (pri-miRNAs) using canonical protein machinery, which includes Drosha/DGCR8 and Dicer, or the non-canonical mirtron pathway. In plant cells, mature miRNAs are excised from pri-miRNAs by the DICER-LIKE1 (DCL1) protein complex. The involvement of multiple regulatory proteins that bind directly to distinct miRNA precursors in a sequence- or structure-dependent manner adds to the complexity of the miRNA maturation process. Here, we present a web server that enables searches for miRNA precursors that can be recognized by diverse RNA-binding proteins based on known sequence motifs to facilitate the identification of other proteins involved in miRNA biogenesis. The database used by the web server contains known human, murine, and Arabidopsis thaliana pre-miRNAs. The web server can also be used to predict new RNA-binding protein motifs based on a list of user-provided sequences. We show examples of miRNAmotif applications, presenting precursors that contain motifs recognized by Lin28, MCPIP1, and DGCR8 and predicting motifs within pre-miRNA precursors that are recognized by two DEAD-box helicases-DDX1 and DDX17. miRNAmotif is released as an open-source software under the MIT License. The code is available at GitHub (www.github.com/martynaut/mirnamotif). The webserver is freely available at http://mirnamotif.ibch.poznan.pl.