Citrus leaf rugose virus (CiLRV) ELISA Kit

The nucleotide sequence of lilac ring mottle ilarvirus (LRMV) RNA 3 consists of 2287 nucleotides and contains two open reading frames (ORF). The first encodes a putative translation product of 285 amino acids (M(r) 31308) and the second encodes a putative translation product of 206 amino acids (M(r) 22751). The 3' terminal nucleotides can be folded into a loop structure similar to models proposed for other ilarviruses, although the last four nucleotides are UCGC not AUGC. The absence of the terminal AUGC motif in both LRMV and two isolates of apple mosaic ilarvirus (ApMV) provides circumstantial evidence which confirms the importance of AUGC moths upstream of the terminal AUGC in the protein binding function associated with these models. Although the 3' terminal structure of LRMV exhibits similarities to that of ApMV, comparison of the putative translation products of the two ORFs with similar products for other ilaviruses showed greatest identity with citrus leaf rugose (CiLRV) and citrus variegation (CVV) ilarviruses both of which are members of subgroup 2 of this genus. Thus it is proposed that LRMV be reassigned to subgroup 2 rather than remaining in its current subgroup, 7, or being reassigned to subgroup 3 which contains ApMV.

Here we describe the complete sequence of RNA 1 and 2 of the WC isolate of tobacco streak virus (TSV). These two sequences complete the information on the genome of TSV, the type member of the genus Ilarvirus, and are the first sequences described for the RNA 1 and RNA 2 of a member of subgroup 1 of this genus. The sequences have a similar organization to those reported for the corresponding RNAs of other ilarviruses. However, the putative translation products of these two molecules differ sufficiently from previously sequenced ilarviruses so that TSV should remain in a subgroup on its own. Phylogenetic comparison of sequence data for RNA 1 with that of other ilarviruses and alfalfa mosaic virus (AMV) reveals two distinct clusters (TSV, CiLRV, and SpLV) and (AMV, PDV, and ApMV). These data support the suggestion [16] based on data for RNA 3 of ilarviruses that AMV should be included as a true ilarvirus.