Ceftazidime [KLH]

Product Overview

Ceftazidime, KLH-conjugate

Target

Ceftazidime

Nature

Synthetic

Tag/Conjugate

KLH

Format

Liquid

Concentration

Approximately 2.0 mg/mL KLH

Buffer

Supplied in 20 mM PBS, pH 7.4

Preservative

None

Storage

Keep below -20°C for up to 1 year. Avoid repeated freeze-and-thaw. For short term storage (< 3 weeks) keep at 4°C.

Introduction

Ceftazidime (INN) is a third-generation cephalosporin antibiotic. Like other third-generation cephalosporins, it has broad spectrum activity against Gram-positive and Gram-negative bacteria. Unlike most third-generation agents, it is active against Pseudomonas aeruginosa, however it has weaker activity against Gram-positive microorganisms and is not used for such infections. Ceftazidime pentahydrate is marketed under various trade names including Cefzim (Pharco B International), Fortum (GSK), and Fortaz.

Antigen Description

The ceftazidime hydrate and KLH (keyhole limpet hemocyanin) (10 mg each) are conjugated by EDC method in 0.1 M MES pH 5.0. The carboxyl group in the ceftazidime is directly linked to an amine group in the KLH, and/or a carboxyl group in the KLH is directly linked to the amine group in the ceftazidime, without any linker by EDC conjugation method. Given the molecular weights of ceftazidime hydrate and KLH are 546.58 Da and 8,000 – 9,000 kDa, respectively, the molar ratio of ceftazidime:KLH in the conjugation solution is 14636 - 16466:1. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS, pH 7.4. The number of ceftazidime that is actually conjugated to each KLH molecule is not determined. Note: Due to its high molecular weight and its tendency to form aggregates, the conjugate is not completely soluble in the buffer that it is in. Therefore, it is strongly recommended to vigorously vortex immediately prior to aliquot or use.

Keywords

cazpentahydrate; gr20263pentahydrate; innersalt,(6r-(6-alpha,7-beta(z)))-droxidpentahydrate; sn401pentahydrate; CEFTAZIME; Kefazim; (6R,7R)-7-[[(2Z)-2-(2-Amino-1,3-thiazol-4-yl)-2-(1-hydroxy-2-methyl-1-oxopropan-2-yl)oxyiminoacetyl]amino]-8-oxo-3-(pyridi