CatCombi Research ELISA Kit

Name: CatCombi Research ELISA Kit
SKU: DEIA2078
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Plasma and Urine

Detection Method

cELISA

Storage

Store the unopened reagents at 2–8°C until expiration date.

Performance Characteristics

Method Comparision
Adrenaline

Noradrenaline

Sensitivity

The lower limit of detection was determined by taking the 2fold standard deviation of the absorbance of the Zero Reference and reading the corresponding value from the standard curve.

General Description

Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress. An increased production of catecholamines can be found with tumours of the chromaffine system (pheochromocytoma, neuroblastoma, ganglioneuroma). An increased or decreased concentration of the catecholamines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The assay kit provides materials for the quantitative measurement of adrenaline and noradrenaline in plasma and urine. Noradrenaline and adrenaline are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline and N-acyladrenaline and then converted enzymatically into N-acylnormetanephrine and N-acylmetanephrine.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Vasopressors and Inotropes in Acute Myocardial Infarction Related Cardiogenic Shock: A Systematic Review and Meta-Analysis

JOURNAL OF CLINICAL MEDICINE

Authors: Karami, Mina; Hemradj, Veemal V.; Ouweneel, Dagmar M.; den Uil, Corstiaan A.; Limpens, Jacqueline; Otterspoor, Luuk C.; Vlaar, Alexander P.; Lagrand, Wim K.; Henriques, Jose P. S.

Abstract

Vasopressors and inotropes are routinely used in acute myocardial infarction (AMI) related cardiogenic shock (CS) to improve hemodynamics. We aimed to investigate the effect of routinely used vasopressor and inotropes on mortality in AMI related CS. A systematic search of MEDLINE, EMBASE and CENTRAL was performed up to 20 February 2019. Randomized and observational studies reporting mortality of AMI related CS patients were included. At least one group should have received the vasopressor/inotrope compared with a control group not exposed to the vasopressor/inotrope. Exclusion criteria were case reports, correspondence and studies including only post-cardiac surgery patients. In total, 19 studies (6 RCTs) were included, comprising 2478 CS patients. The overall quality of evidence was graded low. Treatment with adrenaline, noradrenaline, vasopressin, milrinone, levosimendan, dobutamine or dopamine was not associated with a difference in mortality between therapy and control group. We found a trend toward better outcome with levosimendan, compared with control (RR 0.69, 95% CI 0.47-1.00). In conclusion, we found insufficient evidence that routinely used vasopressors and inotropes are associated with reduced mortality in patients with AMI related CS. Considering the limited evidence, this study emphasizes the need for randomized trials with appropriate endpoints and methodology.

Primary wound care in children

THERAPEUTISCHE UMSCHAU

Authors: Scherer, Cordula; Berger, Steffen

Abstract

Successful wound care of infants and children is facilitated by good preparation and a calm atmosphere.There is not only the child as a patient but also the parents, with their fears and concerns. The physician has to take care of both of them. Parent and child should be informed about the therapeutic intervention appropriately. Reassuring of the child and distraction from the procedure are as important as the treatment itself (e.g. wound stitching or application of wound dressing). Topical anesthesia with LET Get (lidocain, epinephrine, tetracaine), non-stinging methods to clean the wound (NaCl 0,9 %/ Polyhexanid (Prontosan (R)) soaked swabs) and intranasal application of fentanyl/dormicum can be used to avoid fear and pain. Sedation is used deliberately in small children for wound care. Laceration wounds, mainly those affecting the scalp, chin or forehead can be treated in the emergency room without general anesthesia. Extensive wounds, burns and animal bites often require wound care under anesthesia in children.