Anti-Calcitonin Fab monoclonal Antibody, clone H7C2

In the Netherlands, Anacamptis pyramidalis (L.) L.C.M. Rich. is a rare species, which occurs in the calca-reous grass-lands in the Province of Zuid-Limburg and in the calcareous coastal dunes. It is striking that flowering in the dunes is later than inland. Also in other aspects the dune type deviates from the type of the calcareous grasslands such as the colour of the flowers and the shape of the spike. When dune type plants were transplanted to an inland location, the characteristics of the dune type were maintained. These features are therefore not an effect of differences in climate. A number of varieties of Anacamptis pyramidalis has been described. None of these varieties correspond with the dune type and nowhere within the species range such a late flowering time is found. Here, the dune type is described as a new variety: Anacamptis pyramidalis var. dunensis (Dune pyramidal orchid). The variety is characterized by a later flowering time, shorter plants with a mostly shorter more spherical spike and by on an average lighter (light purple pink) coloured flowers. Another character is the more or less S-curved stem.

The aim of the present study was to define the effect of TGF-beta 1 on C2C12 myoblasts myogenesis. TGF-beta 1 together with its receptor is a negative auto-paracrine regulator of myogenesis, which influences the proliferation, differentiation, and functions of muscle cells. TGF-beta 1 exerts highly significant inhibitory effect on differentiation of C2C12 mouse myoblasts manifested by the impairment of cell fusion and very low expression of myosin heavy chain. The study of differentiating C2C12 mouse myoblasts treated with TGF-beta 1 revealed 502 genes (436 down-regulated and 66 up-regulated) with statistically different expression. TGF-beta 1-regulated genes were identified to be involved in 29 biological processes, 29 molecular functions groups and 59 pathways. The strongest inhibiting effect of TGF-beta 1 was observed in the cadherin and Wnt pathways. The key-genes that could play the role of TGF-beta 1 targets during myoblasts differentiation was identified such as: Max, Creb1, Ccna2, Box, MdfI, Tef Tabg1, Cxcl5, Rho, Calca and Lgals4.