CTLA4 (Biotiny)/B7-2 Inhibitor Screening Assay Kit

Intended Use

The CTLA4[Biotinylated]/B7-2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4/B7-2 interaction.

Contents of Kit

1. CTLA4 (CD152), Fc fusion, Biotin-labeled: 3 μg, -80°C;
2. B7-2 (CD86), Fc fusion: 10 μg, -80°C;
3. Streptavidin-HRP: 15 μl, +4°C;
4. 3x CTLA4 Assay Buffer: 50 ml, -20°;
5. Blocking Buffer: 50 ml, +4°C;
6. HRP chemiluminescent substrate A (transparent bottle): 6 ml, +4°C;
7. HRP chemiluminescent substrate B (brown bottle):6 ml, +4°C;
8. White 96-well microplate: 1, +4°C.

Storage

One year from date of receipt when stored as directed.

General Description

B7-2 (CD86) signaling through CTLA4(CD152) has been shown to inhibit T cell activation. This co-inhibitory pathway can be overactive in many tumors, enabling cancers to escape the host's immune system. CTLA4-blocking antibodies, including Ipilimumab (Yervoy) and Tremelimumab, have shown clinical efficacy in treating cancer.