CTLA4/B7-1 (Biotiny) Inhibitor Screening Assay Kit

Intended Use

The CTLA4/B7-1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4/B7-1 signaling.

Contents of Kit

1. B7-1, Biotin-labeled: 5 μg, -80°C;
2. CTLA4: 10 μg, -80°C;
3. Streptavidin-HRP: 15 μl, +4°C;
4. 3x CTLA4 Assay Buffer: 50 ml, -20°C;
5. Blocking Buffer: 50 ml, +4°C;
6. HRP chemiluminescent substrate A (transparent bottle): 6 ml, +4°C;
7. HRP chemiluminescent substrate B (brown bottle): 6 ml, +4°C;
8. White 96-well microplate: 1, +4°C.

Storage

One year from date of receipt when stored as directed.

General Description

The activation of naive T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in an inhibitory signal and prevents T-cell activation. CTLA4:B7-1 interaction is an important drug target for the regulation of the host's response to cancer.