Carnation ringspot virus (CRSV) ELISA Kit

Name: Carnation ringspot virus (CRSV) ELISA Kit
SKU: DEIAPV113
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

host plants

Intended Use

The test can be used to detect CRSV in infected carnation plant.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is moderately high. The virus can be consistently detected in infected plant tissues diluted at 1:270.

General Description

Carnation ringspot virus (CRSV) is a plant pathogenic virus of the family Tombusviridae. A virus with icosahedral isometric particles approximately 34 nm in diameter composed of 180 copies of a Mr 38,000 capsid protein and two genomic ssRNA species of 3.8 and 1.4 kb. The virus was once cosmopolitan in carnations, but is now only infrequently detected due to rigorous detection and sanitation. It also infects grapevine and a number of orchard crops in Central and Eastern Europe. It is readily mechanically transmitted and is also transmitted through the soil without the aid of a biological vector.
Infection in carnations results in diagnostic ringspots, mottling, and leaf and flower distortions . The disease symptoms are enhanced when carnations are co-infected with carnation mottle virus (CarMV; Family Tombusviridae, Genus Carmovirus) (Kemp, 1964; Hollings & Stone, 1965). In severe infections or in susceptible carnation cultivars, leaf tip necrosis can also be observed. In general, CRSV infections do not kill the host plants, but necrosis and other symptoms can become more severe at sustained temperatures between 15 and 20°C. Symptoms are greatly reduced at temperatures above 20°C. Symptoms in stone fruits and other orchard crops may be mild and difficult to discern (Hiruki, 1987). CRSV causes a disease termed "stony pit" in pear (Richter et al., 1978) and decline diseases in sour cherry and apple (Kleinhempel et al., 1980).

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

PARTIAL-PURIFICATION OF A VIRUS-ASSOCIATED WITH A SPANISH ISOLATE OF CITRUS RINGSPOT

PLANT PATHOLOGY

Authors: NAVASCASTILLO, J; MORENO, P; CAMBRA, M; DERRICK, K

Abstract

A citrus ringspot isolate from Star Ruby grapefruit (RS-SR) was mechanically transmitted to Chenopodium quinoa. RS-SR was partially purified by differential centrifugation, fractionation in a sucrose gradient, and agarose gel electrophoresis of selected fractions. Infectivity of concentrated extracts on C. quinoa was lost in individual fractions of the gradient, but it was recovered by combining a top and a bottom component. Both components contained a 48-kDa protein not found in similar preparations from healthy plants. After further purification the 48 kDa protein was detected at the top edge of the agarose gel. In the initial experiments a 38-kDa protein was found in the same fractions that later contained the 48-kDa protein. An antiserum obtained to the 38 kDa protein reacted in Western blots with both the 38- and the 48-kDa proteins, whereas another antiserum raised to the Florida isolate CRSV-4 (also containing a 48-kDa protein) did not react with the 38-kDa protein, indicating that the latter was probably a degradation product of the 48-kDa protein. Filamentous flexous particles were observed by serologically specific electron microscopy in crude extracts from RS-SR-infected C. quinoa plants. These results indicate that RS-SR is associated with a two-component virus similar to those associated with several psorosis and ringspot isolates, and serologically related to CRSV-4.

Queering explanatory frameworks for wartime sexual violence against men

INTERNATIONAL AFFAIRS

Authors: Schulz, Philipp; Touquet, Heleen

Abstract

In this article we argue that prevalent explanatory frameworks of sexual violence against men primarily pursue one line of inquiry, explaining its occurrence as exclusively strategic and systematic, based on heteronormative and homophobic assumptions about violence, gender and sexualities. Feminist IR scholarship has significantly complexified our understanding of conflict-related sexual violence (CRSV), documenting its multiple forms and causes across time and space-thereby moving beyond the persistent opportunism-strategy dichotomy and critically engaging with the dominant 'rape as a weapon of war' narrative. Drawing on empirical material from Sri Lanka and northern Uganda we queer the current explanatory frameworks, analyzing multiple instances of CRSV against men that both simultaneously seem to confirm and defy categorizations as opportunistic or strategic, while being situated in broader and systematic warfare dynamics and unequal power-relationships. Our empirical material shows that relying on crude categorizations such as the opportunism-strategy binary is unproductive and essentialist, as it tends to mask over the complexities and messiness of deeply gendered power relationships during times of war. Binary strategy/opportunism categorizations also imply broader unintended political consequences, including the further marginalization of sexual violence acts that fall outside the dominant scripts or binary frameworks-such as sexual violence against men with opportunistic underpinnings.