Carnation necrotic fleck virus (CNFV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect CNFV in host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is relative high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

General Description

Carnation necrotic fleck virus (CNFV) is a plant pathogenic virus of the family Closteroviridae. The virus causes greyish-white or reddish-purple necrotic flecks, streaks or spots on leaves and stems, with yellowing and complete necrosis on older leaves. Symptoms are milder on younger leaves. Flowers generally do not show symptoms, but owing to the severity of symptoms on leaves and stems, flowers from infected plants are of poor quality and mostly unmarketable. Symptoms can vary according to cultivar and, at least in the Mediterranean area, are particularly severe in spring on young plants. In many cultivars infection can be almost symptomless, especially under cool conditions. Symptoms are greatly increased by mixed infection with other viruses, particularly with carnation mottle carmovirus (Inouye, 1974; Smookler and Loebenstein, 1974; Poupet et al., 1975; Mayhew, 1979; Poncet and Devergne, 1988).

Standard Curve