CDC25A (Phospho-Ser178) ELISA Kit

Name: CDC25A (Phospho-Ser178) ELISA Kit
SKU: DEIA-XYA375
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

cultured cells

Species Reactivity

Human; Mouse; Rat

Detection Method

Indirect ELISA

Intended Use

The CDC25A (Phospho-Ser178) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CDC25A protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated CDC25A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CDC25A phosphorylation.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL (10x)
3. Quenching Buffer: 24 mL (1x)
4. Blocking Buffer: 50 mL (1x)
5. 10x Wash Buffer: 50 mL (10x)
6. 100x Anti-CDC25A (Phospho-Ser178) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-CDC25A Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x)
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x)
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x)
16. Adhesive Plate Seals: 4 seals

Storage

4°C/6 Months

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Caffeic acid 3,4-dihydroxyphenethyl ester arrests colorectal cancer cells growth by upregulating phosphorylation of p38 MAPK

ACTA PHARMACOLOGICA SINICA

Authors: Xin, Wen-xiu; Zhang, Yan-hua; Xie, Xin; Tong, Ying-hui; Zhang, Zhi-zhen; Lian, Xiao-yuan

Abstract

Calebin-A induces cell cycle arrest in human colon cancer cells and xenografts in nude mice

JOURNAL OF FUNCTIONAL FOODS

Authors: Liou, Wen-Shiung; Lin, Chen; Lee, Pei-Sheng; Kalyanam, Nagabhushanam; Ho, Chi-Tang; Pan, Min-Hsiung

Abstract

Calebin-A is a curcuminoid compound isolated from turmeric. Herein, we investigated the antiproliferative effect of calebin-A in human colon cancer cells and demonstrated that calebin-A had a more potent inhibition on cell growth compared with curcumin and tetrahydrocurcumin. In this study, calebin-A caused G(2)/M cycle arrest through decreasing the protein levels of cdc25A, cyclin B, cyclin A and cdc2 and increasing cyclin-dependent kinases inhibitors (CDKI) such as p53 and p21. Moreover, calebin-A increased reactive oxygen species (ROS) levels, triggered a DNA damage response and up-regulated the phosphorylation of H(2)AX, chk1, chk2. Furthermore, intraperitoneally administrated calebin-A on HCT116 tumour xenografts in vivo significantly decreased tumour volumes as well as reduced tumour sizes and proliferating cell nuclear antigen (PCNA) protein levels. These results have an important implication for using calebin-A as a functional food ingredient for cancer prevention. (C) 2016 Elsevier Ltd. All rights reserved.