Anti-CD79A monoclonal antibody

Lung cancer is the first leading cause of cancer-related death in the United States. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer and is associated with a poor patient prognosis. Identification of promising molecular targets is required for the effective prevention and therapy of NSCLC. Epithelial-specific ETS-1 (ESE-1) belongs to the superfamily of ETS transcription factors. The effect of ESE-1 on tumorigenesis is controversial in several types of cancer while its role in lung cancer remains unknown. The present study was designed to investigate whether ESE-1 expression affects tumorigenic activity using human NSCLC cells and a mouse xenograft model. ESE-1 expression suppressed anchorage-independent growth in soft agar assay and led to an increase in G1 arrest and apoptosis in human NSCLC cells. ESE-1 expression suppressed the invasion and migration of human NSCLC cells. Western blot analysis, RT-PCR and promoter assay indicated that ESE-1 expression was transcriptionally downregulated by treatment of transforming growth factor (TGF)-, an EMT (epithelial-mesenchymal transition) stimulator. The xenograft study indicated that ESE-1 expression inhibited tumor formation and development. Our data demonstrated that ESE-1 plays a key role as a tumor suppressor in human NSCLC.

Tandem affinity purification coupled with mass spectrometry (TAP-MS) analysis is a powerful biochemical approach to identify protein-protein associations. Here we describe two datasets generated by a series of TAP-MS analyses to co-purify proteins associated with either ELF3 or ELF4 of the Evening Complex (EC) ("Identification of Evening Complex Associated Proteins in Arabidopsis by Affinity Purification and Mass Spectrometry" (Huang et al., 2016a) [1]) or proteins associated with PCH1, which is a newly identified output of the circadian clock to regulate photoperiodic growth in Arabidopsis thaliana ("PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis" (Huang et al. 2016b) [2]). We used either ELF3, ELF4 or PCH1 fused to a C-terminal tandem affinity tag (6xHis-3xFLAG) as baits and conducted purifications in various genetic mutant backgrounds. These data are discussed in recent publications [1'2], and are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002606 (for EC) and PRIDE: PXD003352 (for PCH1). (C) 2016 The Authors. Published by Elsevier Inc.