Human CBR3 blocking peptide

The sensitization of the chain redox reaction in gamma-irradiated solid films of poly(vinyl chloride) (PVC), containing N,N,N',N'-tetramethyl-4,4'-diaminodiphenylmethane (Am), CBr4 and naphthalene, was investigated by the electron spin resonance and optical spectroscopy methods. The ESR spectra of the gamma-irradiated PVC films at 77 K with the additives of Am, CBr4 and naphthalene were studied. The effects of radiation dose and annealing temperature on the transformation radicals were investigated. The spectra of radicals, taking part in the chain reaction, were separated and identified. The radiation-chemical yields of these radicals and their thermal stability were determined. The dependencies of radical concentration on the annealing temperature for samples in the presence and in absence of naphthalene were compared. It was established that the addition of naphthalene up to 0.2 kmol/m(3) results in an increase of the chain reaction yield from 80 to 150 particle/100 eV in the case if the samples are irradiated at 300 K. If the irradiation is made at 77 K, the yield of oxidized amine Am+ after annealing at 293 K rises from 20 to 45 particle/100 eV as a result of action of naphthalene. The analysis of ESR spectra, made by a computer subtraction, showed that the radiation-chemical yield of free radicals Am-., initiating the chain reaction, is not changed in consequence of the addition of naphthalene. Therefore we suggest that the sensitizing activity of naphthalene is caused by increasing the polymer chain flexibility and, consequently, the diffusion coefficient of active CBr3. radicals, the chain propagating species. (C) 2003 Elsevier B.V. All rights reserved.

Validation of associations for SNPs in RAC2, NCF4 and SLC28A3, identification of a novel association with a TOP2B SNP and screening 23 SNPs putatively relevant to anthracycline-induced cardiotoxicity. Patients & methods: A total of 166 breast cancer patients treated with doxorubicin underwent echocardiogram, including 19 cases with systolic dysfunction (ejection fraction <55%) and 147 controls. Four high priority SNPs were tested in the primary analysis, with appropriate statistical correction, and 23 additional SNPs were screened in an uncorrected secondary analysis. Results: Previously reported associations for RAC2, NCF4 and SLC28A3 could not be validated and a novel association with TOP2B was not discovered in this cohort (all p > 0.05), likely due to inadequate power. Two SNPs were identified in the uncorrected secondary analysis including a protective SNP in ABCB1 (3435C> T, p = 0.049) and a risk allele in CBR3 (V244M, p = 0.012). Conclusion: The associations reported in prior publications and those discovered in this secondary analysis require further replication in independent cohorts.